Live-cell Imaging with Multidimensional Time-lapse

The VivaView LCV110U enables fluorescence imaging of the intrinsic dynamics of living cells in culture. It provides the ability to take multidimensional time-lapse images with complete control over position, wavelength and acquisition. Multiple cells and multiple conditions can therefore be observed simultaneously, improving the accuracy of comparisons and experiments.

Cell Imaging Inside an Incubator

Time-lapse imaging over days or weeks is made possible by integrating an optical microscope with a CO2 incubator.

Parallel Time-lapse Observation of Eight Dishes

Eight ø35 mm glass bottom dishes can be imaged in parallel. Multiple experiments with different cell types/ chemical reagents/ genetic manipulation can be carried out in the same environment. Time-lapse, multicolor fluorescence, Z-stack and transmitted light images can be obtained at multiple locations within each dish.

Eliminates Thermal Drift during Long-term Time-lapse Imaging

LCV110U eliminates focus drift by keeping the objective at 37 °C, the same temperature as inside the incubator. This minimizes the temperature difference that is responsible for changes in focus.

Stress-free Time-lapse Imaging

Experimental settings can easily be controlled. Effortless imaging is achieved through a simple, on-screen control panel. Without the need to continuously monitor cells and correct focus, long-term time-lapse observation can be executed with ease. Complicated accessory components are NOT necessary; all the equipment required for live-cell imaging is fully integrated.

Efficiency Improvements with Parallel Imaging

Multidimensional time-lapse observation can be executed at multiple locations within each ø35 mm glass bottom dish. Moreover, eight ø35 mm glass bottom dishes can be set on the tray, enabling eight different experiments in parallel.

Superior Position Reproducibility

A high-performance motorized stage ensures accurate position reproducibility.

High-contrast DIC Imaging

High-contrast DIC images can be acquired through the optimized optical system.

Clear and High-contrast Fluorescence Imaging

The high-NA objective (40x, NA 0.95) efficiently detects dim fluorescent signals.

A Software Solution for Remote Observation

Once experiments are set up, image acquisition and setting adjustments can easily be made from a network computer. Thus continuous monitoring of the system from within the laboratory is not necessary during a long-term time-lapse experiment.

Loading Reagents During Experiments (Optional)

During observation, chemical solutions can be added to the dishes through a small window on the inner door of the incubator. This process is performed without opening the door and removing the dish, thus minimizing the environmental impact on the sample.