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NBL-6 dermal fibroblast cells were fixed with paraformaldehyde, permeabilized, and treated with a mixture of rabbit (anti-giantin) and mouse (anti-vimentin; pan) primary antibodies, followed by secondary antibodies conjugated to Oregon Green 488 and Texas Red, respectively. Cell nuclei were counterstained with the DNA-selective bisbenzimide dye, Hoechst 33342.
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