Life Science Solutions
Transformed Chicken Embryo Fibroblast Cells (UMNSAH/DF-1 Line)
In a double immunofluorescence experiment, a log phase adherent monolayer culture of chicken embryo fibroblast cells was fixed, permeabilized, blocked with 10 percent normal goat serum, and treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Texas Red and Alexa Fluor 488, respectively. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin.
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