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The BHK-21 fibroblast cell line was established in March of 1961 by I. A. Macpherson and M. G. P. Stoker. The widely used line is a subclone (clone 13) of a parental line derived from the kidneys of five unsexed, 1-day-old hamsters. These hamsters were of the species Mesocricetus auratus and are commonly known as Syrian golden hamsters. Subsequent to 84 days of continuous cultivation, interrupted only for an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation.
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