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In order to label the intermediate filaments in the log phase adherent FoLu culture shown in this confocal image, the fixed and permeabilized cells were blocked and treated with mouse anti-vimentin (porcine eye lens) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 488. Cell nuclei were counterstained with the red-absorbing dye TO-PRO-3.
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