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The GMMe cell line was established by the stable transfection of endometrial tissue of an adult female mink (Mustela vison) using a plasmid vector encoding the simian virus 40 (SV40) large T-antigen driven by the human beta-actin promoter. The cells were cotransfected with a second plasmid vector to confer neomycin resistance and were selected in medium containing G418.
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