FV1200 Laser Scanning Microscopes
Discontinued Products
This product has been discontinued, check out our current product
Imaging of living tissues demands the highest levels of sensitivity which allows for reduced laser power, phototoxicity and photobleaching, and innovative approaches to measurement of fluorescent indicators. The new FV1200 offers increased scanhead light throughput, cooled high sensitivity detector technology, and capitalizes on the advantages of the recently introduced IX83 automated inverted microscope platform. Enhancements in detector and coating technology allow for up to five simultaneous fluorescent detection channels. The industry’s first dedicated laser light stimulation scanner (SIM Scanner) achieves simultaneous stimulation and imaging for real-time visualization of rapid cell responses. This coordination of laser stimulation and imaging makes the FV1200 an ideal choice for FRAP, FLIP and photoactivation.
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Características
Precise, Bright Imaging With Minimum Phototoxicity
The FLUOVIEW FV1200 offers excellent precision, enhancing sensitivity and stability with an advanced optical system, scanners and detection system. In combination with the IX83 microscope, the FV1200 provides users with new opportunities in live cell imaging.
Microscope Frames
Upright Frame
The FV1200 upright frame is a dedicated solution to perform three-dimensional fluorescence imaging for the specimen including the tissue section. Providing space for large samples, a high degree of motorization and nosepiece focus control enables the stage and your sample to remain fixed and stable.
Learn more about upright frame
Inverted Frame
The FV1200 inverted frame solution is dedicated to perform Live Cell Imaging. It's high rigidity structure significantly reduces the impact of heat and vibration and improves the quality of time-lapse imaging. Integration with the IX3-ZDC Z drift compensator enables imaging without focus drift or misalignment.
Learn more about inverted frame
Laser Systems
The multi-combiner enables combinations with all of the following diode lasers: 405 nm, 440 nm, 473 nm, 559 nm and 635 nm. The system can also be equipped with conventional Multi-line Ar laser and HeNe (G) laser.
Dual Type
The multi-combiner outputs laser light with two fibers. Light can be used both for observation and photostimulation.
Single Type
Single channel laser output. AOTF is standard equipment.
Scanners and Detection System
Choice of Main Scanner
Select the scanner to match the purpose at hand, with a choice of a spectral scan unit that achieves 2 nm resolution for high-precision spectroscopy, and a filter scan unit incorporating high-quality filters.
Click here for the details on FV1200 Spectral Version Scan Unit
Click here for the details on FV1200 Filter Version Scan Unit
High-performance Detection System
High-performance, high S/N ratio and superior optical quality are achieved through the smooth integration of a pupil projection lens, a high performance photomultiplier tube, silver-coated galvanometer scanning mirrors with high reflectance across a broad range of wavelengths, and an analog processing circuit that reduces noise to an absolute minimum.
Furthermore, because the system enables image acquisition of this quality with only minimal laser power, phototoxicity is significantly reduced.
High-sensitivity Detector
A high-sensitivity detector employing gallium phosphide (GaAsP) is also available as an option.
Dedicated Scanner for Photostimulation
Combining the main scanner with a photostimulation scanner provides essential flexibility for tracking the diffusion or transport of fluorescently labeled molecules, or for marking specific live cells. The dual-fiber laser combiner also makes it possible to use imaging lasers for photostimulation.
Optical System
UIS2 Objectives
Olympus UIS2 objectives offer world-leading, infinity-corrected optics that deliver unsurpassed optical performance over a wide range of wavelengths.
High S/N Ratio Objectives with Suppressed Autofluorescence
Olympus offers a line of high numerical aperture objectives with improved fluorescence S/N ratio, including objectives with silicone immersion, exceptional correction for chromatic aberration, total internal reflection fluorescence (TIRF), and oil- and water immersion objectives.
Precision and Reliability Meets Deep 3D Imaging
Objectives Deliver High Resolution at Depth
High-resolution Silicone Immersion Objectives
Silicone immersion objectives can be designed with a larger numerical aperture (NA) than water immersion objectives, increasing image resolution and brightness.
Complete the Range with UPLSAPO40XS, 60XS2 and 100XS
Those new objectives from intermediate magnification to high magnification with high NA performances support continuous focus with the IX3-ZDC. Discover continuous high-resolution observation during extended time-lapse imaging.
Enhance the Reliability of Colocalization Analysis, With a Low Chromatic Aberration Objective
Acquire and Analyze Colocalization Imaging with the PLAPON60XOSC
This oil immersion objective minimizes lateral and axial chromatic aberration in the 405-650 nm spectrum, while supporting the reliable acquisition and measurement of colocalization images with superior positional accuracy. The objective also compensates for chromatic aberration through near infrared up to 850 nm, making it an optimal choice for near infrared fluorescence observation.
Maintain High-precision Focus through Extended Time-lapse Imaging
The IX3-ZDC Z Drift Compensator Offers a Range of Functionality for Autofocus
The IX3-ZDC uses low phototoxicity IR light to detect the correct focus position as set by the user. One-shot AF mode allows several focus positions to be set as desired for deeper samples, enabling efficient Z-stack acquisition in multiposition experiments. Continuous AF mode keeps the desired plane of observation precisely in focus, avoiding focus drift caused by temperature changes due to perfusion or reagent addition. This makes it ideal for measurements such as TIRF that require more stringent focusing.
ZDC One-shot Function Detects Focus Fast, Even in High Magnification Observation
IX3-ZDC focus detection and tracking can be performed via the innovative touch panel, independent of software. There’s also a focus search function supported by a cell-safe, near-infrared laser enabling instant focusing on samples and to start scanning.
Tackle the Conflicting Requirements of Expandability and Rigidity with the IX3
A Z-drive guide installed near the revolving nosepiece combines high thermal rigidity with the further stability of a wraparound structure to significantly reduce the impact of heat and vibration and improve the quality of time-lapse imaging. Integration with the IX3-ZDC Z drift compensator permits imaging without focus drift or misalignment, even during temperature changes due to the addition of reagents or a perfusion device. Furthermore, combination with a motorized stage that enables multi point registration allows the achievement of high precision multi point time-lapse imaging.
Olympus’ Unique Super Resolution Technology
FV-OSR (Olympus Super Resolution) technology
Olympus’ widely applicable super resolution method requires no special fluorophores, and can work for a wide range of samples in combination with a large selection of superior optics and high sensitivity detectors. Ideal for colocalization analysis, sequential or simultaneous acquisition of 2 fluorescent signals can lead to resolution of up to 120nm, nearly doubling the resolution of typical confocal microscopy. Operation is simple, with minimal training requirements, and can be added to any FV1000 or FV1200 confocal system, making FV-OSR a truly accessible method for achieving super resolution.
User-Friendly Software
Configurable Emission Wavelength
Emission wavelength can be selected by a choice of the dyne name to set the optimal filters and laser lines.
Wide Choice of Scanning Modes
Diverse scanning modes including ROI, point and high-speed bidirectional scanning can be set by the easy control window. In addition, a configured condition that is previously used can be applied to a new or subsequent experiments.
Adjustable Excitation Laser Power
Easily adjust the optimum laser power for each specimen (live cells and fixed specimens).
Image Acquisition by Application
User-friendly icons offer quick access to functions, for image acquisition according to the application (XYZ, XYT, XYZT, XYλ, XYλT).
Time Controller
Precisely synchronizes different experimental protocols including FRAP, FLIP and FRET by acceptor photobleaching and time-lapse. Save and open settings for later use.
Dark Application Skin
The Dark Application Skin minimizes the influence of the noise from the screen to the detecting senors by darkening the control screen.
Multi-Dimensional Time-Lapse
Multi-dimensional Time-lapse Imaging with Outstanding Positional Accuracy
The FLUOVIEW FV1200 can be used for ideal multi-dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and IX3-ZDC Z drift compensator.
Simultaneous Photostimulation
Combined Photostimulation and Imaging with Microsecond Precision Control
The SIM scanner system combines the main scanner with a photostimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous imaging of the effects of stimulation on the cell.
FLIP - Fluorescence Loss in Photobleaching
Fluorescence loss in photobleaching (FLIP) combines imaging with continuous bleaching of a specific region to observe the diffusion of a target protein within a cell. The changes in the image over time make it possible to observe the location of structural bodies that inhibit the diffusion of the molecule.
FRAP - Fluorescence Recovery after Photobleaching
Exposure of fluorescent-labeled target proteins to strong laser light causes their fluorescence to fade locally. Fluorescence recovery after photobleaching (FRAP) is used to observe the gradual recovery of fluorescence intensity caused by protein diffusion from the area surrounding the bleached region. By examining the resulting images, it is possible to characterize the diffusion speed of the molecule, and the speed of binding and release between the molecule and cell structures.
Uncaging
A 405 nm laser is optional for uncaging with the SIM scanner system. Caged compounds can be uncaged point-by-point or within a region of interest, while the main scanner of the FV1200 captures images of the response with no time delay.
Multi-Stimulation Software Enables High Speed Multi point Scans and Mapping Scans
Users can designate the number of points on an image for light stimulation. Stimulation timing, duration and interval can be defined in a magnitude of μs and the user can program the experiment with continuous or pulse stimulation. The same software also provides features that allow extended multiple points surrounding one single point to cover a small area.
Light stimulation can be applied to a rectangular region of interest. Software control of stimulation of each point assures neighboring points will not be excited. This allows the user to observe reaction of sample more accurately. Changes in intensity from those points can be processed as a mapped image or graph.
Diffusion Measurement Package
Diffusion Measurement Package Extends Analytical Capabilities
This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds.
Accessory Units That Support an Array of Applications
Illumination Units
Conventional illumination modules are designed for long-duration time-lapse experiments. Since light is introduced through fiber delivery systems, no heat is transferred to the microscope.
Fluorescence Illumination Source/U-HGLGPS
The pre-centered fluorescence illumination source requires no adjustment and has an average lifespan of 2,000 hours.
Transmitted Light Detection Unit
External transmitted light photomultiplier detector and 100 W Halogen conventional illumination, integrated for both laser scanning and conventional transmitted light
Nomarski DIC observation. Motorized exchange between transmitted light illumination and laser detection. Simultaneous multi-channel confocal fluorescence image and transmitted DIC acquisition enabled.
Optional Upgrade Equipment for FV1200
4th Channel Detector Unit
Attaches to the optional port of either the filter or spectral type scanning unit and is used as a 4th confocal fluorescence detection channel. This is a filter-based fluorescence detection unit.
Fiber Port for Fluorescence Output
Confocal fluorescence emission can be introduced via a fiber delivery system into an external device. The fiber port equipped with FC connector (fiber delivery system not included).
TIRF Unit
Enables control of the necessary volume of excitation light using FV1200 software. This unit enables TIRF imaging using the confocal laser light source.
Especificações
| Laser Unit > Automatic Introduction Optic |
AOTF (Single or dual fiber type)
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| Laser Unit > Optional Visible Light Laser for Stimulation | - | |
| Laser Unit > LD Laser for Visible Light > 405 nm |
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| Laser Unit > LD Laser for Visible Light > 440 nm |
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| Laser Unit > LD Laser for Visible Light > 473 nm |
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| Laser Unit > LD Laser for Visible Light > 559 nm |
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| Laser Unit > LD Laser for Visible Light > 635 nm |
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| Laser Unit > Multiline Argon Laser (457 nm, 488 nm, 515 nm, Total 30 mW) |
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| Laser Unit > HeNe(G) Laser (543 nm, 1 mW) |
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| Scanning and Detection > Main Scanner > Standard Laser Ports |
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| Scanning and Detection > Main Scanner > Detector > Standard |
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| Scanning and Detection > Main Scanner > Detector > Cooled GaAsP-PMT 2 CH |
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| Scanning and Detection > Main Scanner > Detector > Optional 4 CH |
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| Scanning and Detection > Main Scanner > Photo Detection Method > Analog Integration |
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| Scanning and Detection > Main Scanner > Photo Detection Method > Hybrid Photon Counting |
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| Scanning and Detection > Main Scanner > VIS - UV - IR Excitation Dichromatic Mirror Turret |
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| Scanning and Detection > Main Scanner > Beamsplitter Turrets |
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| Scanning and Detection > Main Scanner > Wavelength Selection |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Galvanometer Mirror Scanner (X, Y) |
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 2D |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 3D |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 4D |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 5D |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > Other | Line scanning: Straight line with free orientation, free line, Point scanning | |
| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Speed |
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Pinhole |
<Spectral type>
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| Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Zoom |
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| Scanning and Detection > Field Number (NA) |
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| Scanning and Detection > Z-Drive |
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| Scanning and Detection > Transmitted Light Detector Unit | Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope frame | |
| Microscope > Frame |
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| Microscope > Frame > Inverted |
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| Microscope > Objectives and Focus > Oil Supply |
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| Microscope > XY Stage > Specimen Holder |
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| Microscope > Incubator > Room Environment |
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| Microscope > Incubator > Heating Method |
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| System Control > Controller |
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| System Control > Power Supply Unit |
<Spectral type>
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| Optional Unit > SIM Scanner |
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| Optional Unit > TIRFM Unit |
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| Software > Image Acquisition |
Normal scan:
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| Software > Programmable Scan Controller | Time Controller function | |
| Software > 2D Image Display | Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input | |
| Software > 3D Visualization and Observation |
Interactive volume rendering: volume rendering display, projection display, animation displayed (save as OIF, AVI or MOV format)
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| Software > Image Format |
OIB/ OIF image format
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| Software > Spectral Unmixing | 2 Fluorescence spectral unmixing modes (normal and blind mode) | |
| Software > Image Processing |
Filter type: Sharpen, Average, DIC Sobel, Median, Shading, Laplacian
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| Software > Image Analysis | Fluorescence intensity, area and perimeter measurement, time-lapse measurement | |
| Software > Statistical Processing | 2D data histogram display, colocalization | |
| Software > Optional Software |
Review station software, Off-line FLUOVIEW software for date analysis.
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| Software > Image Acquisition Mode | - | |
| Software > Map Image Acquisition | - | |
| Software > Multi Area Time-Lapse | - | |
| Software > Image Acquisition Area | - | |
| Software > Image Display |
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| Software > Cross Talk Reduction | - | |
| Software > Acquisition Image File Type |
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| Software > Image File Type Available for Viewing |
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| Software > Image Editing | - | |
| Software > 3D Image Construction | - | |
| Software > Basic Features | - | |
| Software > IR Laser Controlling |
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| Software > Optional Motorised-Stage Software | - | |
| Software > Optional Mapping and Multiplepoint Simulation Software | - | |
| Software > Optional Sequencer Manager | - | |
| Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Power Consumption |
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| Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Power Consumption |
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| Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Power Consumption |
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| Dimensions, Weight and Power Consumption > Microscope Control Unit > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Microscope Control Unit > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Microscope Control Unit > Power Consumption |
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| Dimensions, Weight and Power Consumption > FV Power Supply Unit > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > FV Power Supply Unit > Weight (kg) |
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| Dimensions, Weight and Power Consumption > FV Power Supply Unit > Power Consumption |
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| Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Power Consumption |
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| Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Power Consumption |
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| Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Power Consumption |
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| Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Weight (kg) |
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| Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Power Consumption |
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| Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Weight (kg) |
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| Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Power Consumption |
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| Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Dimensions (mm) |
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| Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Weight (kg) |
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| Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Power Consumption |
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| Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Ambient Temperature | - | |
| Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Maximum Relative Humidity | - | |
| Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Supply Voltage Fluctuations | - |