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The unique FLUOVIEW FV10i-DOC is a fully automated confocal laser-scanning microscope. The completely re-engineered design of this microscope, integrated into a self-contained package with a variety of functions, enables even inexperienced and first-time users to perform easily and efficient high-quality confocal imaging. Olympus has made no compromises in ergonomics and image quality, using high-quality optical components and smart and easy software.
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A Unique, Easy-to-use, Self-contained Confocal Laser Scanning Microscope with a Small Footprint
The FV10i features a unique self-contained design. Simplified and optimized, key components, including an incubator and laser combiner, have been integrated into a self-contained package with no compromises. The system, which is easier to set up and use, features the same functionality as a high-end confocal laser scanning microscope while adding vibration isolation and a light tight cover. As a result, the FV10i can be operated by new and experienced users alike and, with a compact footprint, can be installed in any laboratory without the need for a dedicated dark room.
1.Dark Room Free | The Microscope body and light tight cover are integrally combined. The FV10i can be used with ease in a laboratory, unlike conventional confocal laser scanning microscopes which may require a dark room. |
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2.Scanning Unit | The system is equipped with a detector which automatically sets conditions in accordance with fluorescence dye on a scanning unit. Imaging can be performed in the conditions that are most suited for each fluorescence dye. |
3.Microscope Function | The FV10i's excellent optical and mechanical modules are totally integrated. The FV10i can capture images from 10× to 600× magnification with 10×, 60× objectives and optical zoom. |
4.Vibration Isolation Function | Equipped with built-in vibration insulators. A vibration isolation table is not required and so can be installed directly on an experimental tabletop. |
5.Laser Combiner | Equipped with four diode laser units, each unit utilizes a compact diode laser for longer life and power-saving compared to traditional confocal systems. |
Equipped with 4 Diode Lasers
The system is equipped with four (405/473/559/635 nm) lasers. Multi-stained specimens can be imaged with up to four fluorescence dyes. Maintenance-free and power-saving diode lasers with longer operating lives are employed in all the laser units, and operate with low noise levels.
Detector Utilizes a Newly Developed Spectrum Method
The detecting mechanism has two fluorescence channels, and one phase contrast channel. The fluorescent channels use a newly developed spectrum method comprising grating, beam splitter, and slit. In addition, they are equipped with the variable barrier filter function where the most suitable wavelength width is set automatically in accordance with the characteristics of the fluorescence dye.
Two Sequential Modes
The FV10i is equipped with two sequential modes. Images can be acquired through line sequences without crosstalk in imaging with two fluorescence dyes, and with three or four dyes in frame sequences with the virtual channel function.
Objectives of 10× and 60× are System-mounted
The system is equipped with objectives of 10× and 60×. Zoom magnification can be changed continually from 10× to 600×. The most suitable imaging area can be set depending on the size of the specimen.
Stress-free Operation for Every User
Only two manual steps are required of the user: placing of the specimen on the stage and closing the cover. After that, the sophisticated user interface offers clear and efficient operation of the microscope. The selection of the imaging point, for example, can be performed easily using the newly designed image mapping menu without need for special experience or expertise. Furthermore, using advanced functionality that is only found in Olympus products, automatic focus and intensity adjustment allow the imaging conditions to be set up according to the type of specimen and observation mode required. In addition, the system is equipped with a navigation function that identifies the operational step of the imaging procedure and guides the operator to the next appropriate operation. The FV10i confocal laser scanning microscope, therefore, provides a stress-free and comfortable operating environment even for first-time users.
Setting
Place a specimen, and select a fluorescence dye. The FV10i automatically selects the most suitable imaging conditions based on the fluorescence dye selection.
Image Mapping Menu
Just click the <Start> button, and a map image of the specimen is created automatically. Users can then easily identify the desired image acquisition point.
Image Capturing
Through the sophisticated operating software, the image capture area or zoom magnification can be set quickly. Image capture is then completed at the click of a button.
Following the loading of the specimen, a map image can be acquired by simply clicking the <Start> button in the "Acquire Map Image" window. With this bird's-eye view of the specimen, the user can quickly and easily select the imaging area to be captured.
Create Map Area
The map image provides the user with an overview of the possible image capture area. The selectable map areas are automatically displayed in a diagram according to the type of specimen holder that has been used, such as a 35mm diameter dish or glass slide. An area can be selected and map image acquired by clicking on the desired scan area in the diagram. This will display an overview image of the area on the “Map Image” screen. Selecting an alternative area requires just a single click operation.
Fluorescence Dye Selection
A separate map image can be displayed for each fluorescence dye. These images can be displayed individually or be overlaid with each other.
Scanning Setting
One of two scanning orders can be selected depending on the experimental requirements.
Automatic
A map image is automatically acquired from the center outward in a spiral pattern, allowing even a first-time user to easily identify the confocal view area.
Manual
The desired map image areas can be selected directly up to a maximum 9 × 9 area. Manual selection is more efficient than automatic selection as the ROI (Region of Interest) can be narrowed down in advance.
A Map Image of the Specimen Can be Automatically Created
A map image can be created by automatically detecting which type of specimen holder is used. High speed with low resolution or low speed with high resolution image mapping options is available.
The desired imaging region can be selected using the map image and live image screens utilizing the zooming function of the intuitive user interface. The user friendly navigation functions allow even a first-time user to capture images with ease.
Observation Mode Selection |
Five types of observation modes can be selected including time-lapse, Z-stack, and multi-area.
Time-lapse In time-lapse mode, images are continuously acquired at predetermined intervals. Z-stack In Z-stack mode, images are repeatedly acquired in different focus positions. Three-dimensional images can be constructed. Z-stack time-lapse Imaging which integrates Z-stack and time-lapse are acquired. Multi-area time-lapse Time-lapse imaging is performed automatically at pre-selected points. Multi-area Z-stack time-lapse Imaging where all three functions are performed. |
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Multi-area Setting | Register the areas for imaging in multi-area mode. Appropriate imaging conditions can be set for each area. |
Map Image | The image acquired in [Acquire Map Image] is displayed. Specific regions can be selected for closer examination. |
Control Screen |
Imaging conditions can be set in detail with operation of various controllers. Main settings include:
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Live Image | The selected point from the map image screen is displayed. The imaging area is determined through the framing and zooming functions. It is possible here to switch between the displays for each type of fluorescence dye. |
The System is Equipped with a User Friendly Navigation Function
Clicking the <Navigation> button shows the operational procedure and highlights the operational button. Just follow the navigational guidance to easily complete your imaging.
Stitching Function
Wide-angle high-resolution imaging can be obtained by acquiring the adjacent regions. A map image of the entire glass slide can also be created.
Dedicated Olympus software is provided as part of the standard specifications, bringing editing and analysis capabilities to images taken by FV10i.
1.3D Display Function | The FV10i supports the Alpha Blend method and Maximum Intensity Projection method for the 3D display function. Also, the system is equipped with various display functions which allow a user to freely change the angle of 3D images and section the image at any spot. | ||||||||
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2.Easy Image Searching | Thumbnail list is possible on the main screen allowing a user to easily search for previous image data. | ||||||||
3.2D Analysis Tool |
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4.Data Manager | The data manager displays thumbnails and various file information with clarity. |
File Input/Output
OIF (Olympus Image Format) is employed to store various parameter settings and images together. This software supports a wide range of well-used formats with high interchangeability including TIFF, BMP and JPEG.
The system is equipped with an original UPLSAPO–equivalent Olympus objective, which is intended to provide the best fluorescence observation performance available in the world for a 60X objective. The objective has a high numerical aperture of 1.35 enabling high resolution imaging. The FV10i motorized stage automatically moves to the oil supply position when switching to the oil immersion objective, allowing oil to be supplied efficiently without removing the specimen.
Equipped with Specimen Holders
The system is equipped with specimen holders, usable for a dia. 35 mm glass bottom dishes, glass slides, cover glass chambers (8 wells type), and well slides (8 wells type). Specimens can be observed without the risk of contamination using the closed contamination-free plastic cover of a dia. 35 mm glass bottom dish.
Capturing Adjacent Images in Wide field
Imaging of adjacent images is possible to create big mosaic images. These can be captured in high-definition and wide field of view.
HDD Recording for Storing Large Volumes of Data
The microscope comes equipped with a HDD (hard-disk drive) recording function. The images captured are stored automatically in the HDD. Large volumes of data, such as those obtained from long-term time-lapse imaging can be stored. During imaging, editing/analysis of previously taken images is also possible. An external HDD connected to a network can be specified as the destination, and the saved images can be viewed on a remote PC while performing separate imaging.
Laser Unit > Qualified IR Pulsed Laser with Negative Chirp for Multiphoton Excitation | - | |
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Laser Unit > Automatic Introduction Optic |
Built-in
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Laser Unit > Optional Visible Light Laser for Stimulation | - | |
Laser Unit > LD Laser for Visible Light > 405 nm |
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Laser Unit > LD Laser for Visible Light > 440 nm |
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Laser Unit > LD Laser for Visible Light > 473 nm |
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Laser Unit > LD Laser for Visible Light > 559 nm |
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Laser Unit > LD Laser for Visible Light > 635 nm |
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Laser Unit > HeNe(G) Laser (543 nm, 1 mW) |
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Scanning and Detection > Main Scanner > Standard Laser Ports |
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Scanning and Detection > Main Scanner > Detector > Standard |
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Scanning and Detection > Main Scanner > Detector > Cooled GaAsP-PMT 2 CH |
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Scanning and Detection > Main Scanner > Detector > Optional 4 CH |
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Scanning and Detection > Main Scanner > Photo Detection Method > Analog Integration |
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Scanning and Detection > Main Scanner > Photo Detection Method > Hybrid Photon Counting |
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Scanning and Detection > Main Scanner > VIS - UV - IR Excitation Dichromatic Mirror Turret |
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Scanning and Detection > Main Scanner > Beamsplitter Turrets |
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Scanning and Detection > Main Scanner > Wavelength Selection | Variable barrier fi lter mechanism for fl uorescence channel by diffraction grating and slit | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Galvanometer Mirror Scanner (X, Y) |
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 2D | XY | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 3D | XYT, XYZ | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 4D | XYZT | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > 5D | - | |
Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Modes > Other |
Map image, one shot
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Speed |
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Pinhole |
Single motorized
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Scanning and Detection > Galvanometer Scanner (Normal Imaging) > Scanning Zoom |
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Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 2D | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 3D | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 4D | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > 5D | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Modes > other | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Speed | - | |
Scanning and Detection > Resonant Scanner (High-Speed Imaging) > Scanning Zoom | - | |
Scanning and Detection > Field Number (NA) |
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Scanning and Detection > Z-Drive |
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Scanning and Detection > Transmitted Light Detector Unit | Phase Contrast: 1 channel | |
Microscope > Frame |
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Microscope > Frame > Inverted |
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Microscope > Objectives and Focus > Objectives |
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Microscope > Objectives and Focus > Auto Focus (AF) |
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Microscope > Objectives and Focus > Water Supply |
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Microscope > Objectives and Focus > Oil Supply |
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Microscope > XY Stage > XY Driving Method |
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Microscope > XY Stage > Specimen Holder |
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Microscope > Incubator > Room Environment |
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Microscope > Incubator > Heating Method |
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System Control > Controller |
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System Control > Power Supply Unit | - | |
Optional Unit > SIM Scanner |
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Optional Unit > TIRFM Unit |
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Software > Image Acquisition | - | |
Software > Programmable Scan Controller | - | |
Software > 2D Image Display | - | |
Software > 3D Visualization and Observation | - | |
Software > Image Format | - | |
Software > Spectral Unmixing | - | |
Software > Statistical Processing | - | |
Software > Optional Software | - | |
Software > Image Acquisition Mode |
Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area
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Software > Map Image Acquisition | Automatic selection of map image of 3 x 3 – 35 x 7 fields according to 10 X objective lens (The maximum area varies in accordance to the specimen holder used), and manual selection of map acquisition area | |
Software > Multi Area Time-Lapse |
Automatic multi area time-lapse by motorized XY stage
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Software > Image Acquisition Area | Area appointment: All area, clipping square area (minimum area: 96 x 96 pixels) | |
Software > Image Display |
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Software > Cross Talk Reduction | Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel) | |
Software > Acquisition Image File Type |
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Software > Image File Type Available for Viewing |
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Software > Image Editing | LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination | |
Software > 3D Image Construction | 3D display: AlphaBrend method, Maximum intensity projection method 3D animation display, free orientation of cross section display | |
Software > IR Laser Controlling |
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Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Weight (kg) |
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Dimensions, Weight and Power Consumption > Microscope with Scan Unit > Power Consumption |
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Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Weight (kg) |
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Dimensions, Weight and Power Consumption > Fluorescence Illumination Unit > Power Consumption |
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Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Weight (kg) |
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Dimensions, Weight and Power Consumption > Transmitted Light Detection Unit > Power Consumption |
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Dimensions, Weight and Power Consumption > Microscope Control Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Microscope Control Unit > Weight (kg) |
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Dimensions, Weight and Power Consumption > Microscope Control Unit > Power Consumption |
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Dimensions, Weight and Power Consumption > FV Power Supply Unit > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > FV Power Supply Unit > Weight (kg) |
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Dimensions, Weight and Power Consumption > FV Power Supply Unit > Power Consumption |
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Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Weight (kg) |
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Dimensions, Weight and Power Consumption > Power Supply Unit for Laser Combiner > Power Consumption |
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Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Weight (kg) |
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Dimensions, Weight and Power Consumption > Laser Combiner (with Ar Laser Heads) > Power Consumption |
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Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Weight (kg) |
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Dimensions, Weight and Power Consumption > Laser Combiner (without Ar Laser Heads) > Power Consumption |
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Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Weight (kg) |
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Dimensions, Weight and Power Consumption > LD559 Laser Power Supply > Power Consumption |
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Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Weight (kg) |
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Dimensions, Weight and Power Consumption > Multi Ar Laser Power Supply > Power Consumption |
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Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Dimensions (mm) |
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Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Weight (kg) |
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Dimensions, Weight and Power Consumption > HeNe(G) Laser Power Supply > Power Consumption |
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Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Ambient Temperature | 18 - 28 ℃ (flutuação ±2 ℃) | |
Dimensions, Weight and Power Consumption > Operating Environment (Indoor Use) > Maximum Relative Humidity | 30 - 80 % (sem condensação) |
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