Life Science Solutions
Biotin (Vitamin H)
Spherulitic crystallites of the important vitamin biotin (vitamin H) acquire a pseudo three-dimensional appearance when photographed under a combination of polarized light and Hoffman modulation contrast. The photomicrograph above was taken using this combination in addition to a first-order retardation placed between the specimen and the analyzer at a 45-degree angle to the vibration directions of the polarizer and analyzer.
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