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The proximity of the Golgi complex and nuclei in rat enteroglial cells was probed in a double immunofluorescence experiment with mouse anti-histones (pan) and rabbit anti-giantin primary antibodies. The antibody targets were visualized with goat secondary antibodies conjugated to Texas Red and Alexa Fluor 488, respectively, while the actin cytoskeletal framework was labeled with Alexa Fluor 350 conjugated to phalloidin.
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