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A culture of COS-7 fibroblasts was labeled for mitochondria with MitoTracker Red CMXRos, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, a cyclic peptide derived from the toxic death cap fungus (Amanita phalloides). In addition, the fibroblasts were counterstained for DNA in the cell nucleus with Hoechst 33258.
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