Visualizing the Proximity Between the Golgi Complex and the Nucleus in Carcinoma Cell Cultures
The Golgi apparatus was targeted in the culture of human cervical adenocarcinoma (HeLa) cells* appearing in this section by treating the cells with rabbit (anti-giantin) primary antibodies, followed by secondary antibodies conjugated to Alexa Fluor 568. DNA in the cell nucleus was counterstained with the red-absorbing dye TO-PRO-3 (pseudocolored cyan).
*Although it became one of the most important cell lines in medical research, it’s imperative that we recognize Henrietta Lacks’ contribution to science happened without her consent. This injustice, while leading to key discoveries in immunology, infectious disease, and cancer, also raised important conversations about privacy, ethics, and consent in medicine.
To learn more about the life of Henrietta Lacks and her contribution to modern medicine, click here.
http://henriettalacksfoundation.org/
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