Indian Muntjac Deer Skin Fibroblast Cells
A log phase monolayer culture of Indian Muntjac cells was fixed, permeabilized, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of goat anti-rabbit secondary antibody fragments (heavy and light chain) conjugated to Cy2. In addition, the culture was labeled for the filamentous actin network with Alexa Fluor 568 conjugated to phalloidin, and for DNA with Hoechst 33342.
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