Indian Muntjac Deer Skin Fibroblast Cells
A culture of Indian Muntjac fibroblasts was fixed, permeabilized, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with secondary antibody fragments (heavy and light chain) conjugated to Cy2. In addition, the culture was labeled for mitochondria with MitoTracker Red CMXRos, and for DNA with Hoechst 33342.
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