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Using the popular combination of MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, an adherent culture of EGC cells was first incubated with the MitoTracker, and then fixed, permeabilized and blocked with bovine serum albumen. The specimen was subsequently labeled with the phallotoxin conjugate, followed by the DAPI counterstain.
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