Mongoose Skin Fibroblast Cells (APM)
Applying a collection of popular mitochondrial, actin, and DNA probes, a culture of APM cells was grown to log phase, treated with MitoTracker Red CMXRos before fixing, and then labeled with phalloidin and Hoechst 33258 after permeabilization. The red fluorescence arises from the mitochondrial dye (MitoTracker), while the phalloidin was conjugated to Alexa Fluor 488 to generate green fluorescence. Nuclei are rendered in light blue.
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