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The popular multiple fluorophore combination of MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and Hoechst 33258 was employed to perform a triple label experiment on a monolayer of African water mongoose cells. The trio of fluorophores stains the cells with red (mitochondria), green (filamentous actin), and blue (DNA in the nucleus) fluorescence emission.
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