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Elements of the cytoskeletal and microtubule networks were imaged in a Vero cell culture by treating the fixed and permeabilized cells with mouse anti-alpha-tubulin primary antibodies, followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568. Mixed with the secondary antibody cocktail was Alexa Fluor 350 conjugated to phalloidin to target filamentous actin. Nuclei were counterstained with SYTOX Green.
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