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The monolayer culture of Indian Muntjac fibroblast cells shown in this section was treated with MitoTracker Red CMXRos in medium containing 15 percent Cosmic calf serum, fixed with the same medium containing 3.7 percent paraformaldehyde, permeabilized with 0.2 percent Triton X-100, and then counterstained with DRAQ5 (pseudocolored cyan), targeting DNA in the cell nuclei.
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