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Investigating Spheroid Architecture Using the FV3000 Confocal Microscope 

Phenotypic tumour heterogeneity arising due to differentially cycling cell populations has been implicated in increased therapy resistance. This phenomenon cannot be assessed in adherent cell culture, where microenvironmental conditions are homogeneous. Thus, we utilise melanoma spheroids to model the 3D tumour microenvironment including the extracellular matrix (ECM) and study spheroid structure, necrotic region, individual cell arrangement within and gene expression patterns. We achieve this by exploiting the fluorescence ubiquitination cell cycle indicator (FUCCI) system to monitor cell cycle stages as a surrogate marker for phenotypic tumour heterogeneity, tissue clearing and confocal microscopy using FV3000.


関連製品

共焦点レーザー走査型顕微鏡

FV3000

  • ガルバノスキャナーのFV3000と、ガルバノ/レゾナントのハイブリッドキャナーのFV3000RS
  • 高感度・高精度のTruSpectral分光検出器
  • 新開発マルチチャンネルモードによる16チャンネルのアンミキシング

Investigating Spheroid Architecture Using the FV3000 Confocal Microscope

Phenotypic tumour heterogeneity arising due to differentially cycling cell populations has been implicated in increased therapy resistance. This phenomenon cannot be assessed in adherent cell culture, where microenvironmental conditions are homogeneous. Thus, we utilise melanoma spheroids to model the 3D tumour microenvironment including the extracellular matrix (ECM) and study spheroid structure, necrotic region, individual cell arrangement within and gene expression patterns. We achieve this by exploiting the fluorescence ubiquitination cell cycle indicator (FUCCI) system to monitor cell cycle stages as a surrogate marker for phenotypic tumour heterogeneity, tissue clearing and confocal microscopy using FV3000.


関連製品

共焦点レーザー走査型顕微鏡

FV3000

  • ガルバノスキャナーのFV3000と、ガルバノ/レゾナントのハイブリッドキャナーのFV3000RS
  • 高感度・高精度のTruSpectral分光検出器
  • 新開発マルチチャンネルモードによる16チャンネルのアンミキシング
Experts
Ms. Gency Gunasingh
Research Assistant
The University of Queensland Diamantina Institute

Ms. Gency Gunasingh completed her Master of biotechnology degree from University of Queensland in 2012 and did her Masters project under Dr. Andrew Prowse and Prof. Peter Gray at Australian Institute for Bioengineering and Nanotechnology. Her primary area of research was large scale generation of cardiac progenitors from embryonic stem cells in 3D. She then worked under Prof. Brian Gabrielli at UQ Diamantina institute on developing 3D tumour spheres in melanoma for in vitro drug testing. She currently works for Prof. Nikolas Haass at UQDI on understanding tumour heterogeneity and tumour architecture in melanoma spheroids.

Investigating Spheroid Architecture Using the FV3000 Confocal Microscope2024年12月22日
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