Section Overview:
By directly coupling the advanced microscopic technique of fluorescence resonance energy transfer (FRET) to the ubiquitous green fluorescent protein and its multispectral variants fused with selected biopolymers, ingenious researchers have created several new classes of biosensors capable of elucidating mechanisms of signaling, enzymatic cleavage, and other critical functions in living cells. This section features a bibliography of literature sources for review articles and original research reports on the construction, applications, and continued development of biosensor fluorescent proteins.
Literature Sources
- Abad, M. F. C., DiBenedetto, G., Magalhães, P. J., Filippin, L., and Pozzan, T., Mitochondrial pH monitored by a new engineered green fluorescent protein mutant., Journal of Biological Chemistry 279: 11521-11529 (2004). | PubMed |
- Abedi, M. R., Caponigro, G., and Kamb, A., Green fluorescent protein as a scaffold for intracellular presentation of peptides., Nucleic Acids Research 26: 623-630 (1998).
| PubMed | - Allen, G. J., Chu, S. P., Schumacher, K., Shimazaki, C. T., Vafeados, D., Kemper, A., Hawke, S. D., Tallman, G., Tsien, R. Y., Harper, J. F., Chory, J., and Schroeder, J. I., Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant., Science 289: 2338-2342 (2000). | PubMed |
- Allen, G. J., Kwak, J. M., Chu, S. P., Llopis, J., Tsien, R. Y., Harper, J. F., and Schroeder, J. I., Cameleon C\calcium indicator reports cytoplasmic calcium dynamics in Arabidopsis guard cells., The Plant Journal 19: 735-747 (1999). | PubMed |
- Ataka, K. and Pieribone, V. A., A genetically targetable fluorescent probe of channel gating with rapid kinetics., Biophysical Journal 82: 509-516 (2002). | PubMed |
- Awais, M., Sato, M., Sasaki, K., and Umezawa, Y., A genetically encoded fluorescent indicator capable of discriminating estrogen agonists from antagonists in living cells., Analytical Chemistry 76: 2181-2186 (2004). | Anal Chem |
- Baird, G. S., Zacharias, D. A., and Tsien, R. Y., Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral., Proceedings of the National Academy of Sciences (USA) 97: 11984-11989 (2000). | PubMed |
- Barondeau, D. P., Kassman, C. J., Tainer, J. A., and Getzoff, E. D., Structural chemistry of a green fluorescent protein zinc biosensor., Journal of the American Chemical Society 124: 3522-3524 (2002). | PubMed |
- Bartlett, P. J., Young, K. W., Nahorski, S. R., and Challiss, R. A. J., Single cell analysis and temporal profiling of agonist-mediated inositol 1,4,5-trisphosphate, Ca2+, diacylglycerol, and protein kinase C signaling using fluorescent biosensors., The Journal of Biological Chemistry 280: 21837-21846 (2005). | PubMed |
- Bastiaens, P. I. H. and Pepperkok, R., Observing proteins in their natural habitat: The living cell., Trends in Biochemical Sciences 25: 631-637 (2000). | PubMed |
- Berridge, M. J., Lipp, P., and Bootman, M. D., The versatility and universality of calcium signaling., Nature Reviews Molecular Cell Biology 1: 11-21 (2000). | PubMed |
- Billinton, N., Barker, M. G., Michel, C. E., Knight, A. W., Heyer, W. D., Goddard, N. J., Fielden, P. R., and Walmsley, R. M., Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor., Biosensors and Bioelectronics 13: 831-838 (1998).
| PubMed | - Biondi, R. M., Baehler, P. J., Reymond, C. D., and Véron, M., Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum., Nucleic Acids Research 26: 4946-4952 (1998). | PubMed |
- Bozza, T., McGann, J. P., Mombaerts, P., and Wachowiak, M., In vivo imaging of neuronal activity by targeted expression of a genetically encoded probe in the mouse., Neuron 42: 9-21 (2004). | PubMed |
- Bracha-Drori, K., Shichrur, K., Katz, A., Oliva, M., Angelovici, R., Yalovsky, S., and Ohad, N., Detection of protein-protein interactions in plants using bimolecular fluorescence complementation., The Plant Journal 40: 419-427 (2004). | PubMed |
- Bünemann, M., Frank, M., and Lohse, M. J., Gi protein activation in intact cells involves subunit rearrangement rather than dissociation., Proceedings of the National Academy of Sciences (USA) 100: 16077-16082 (2003). | PubMed |
- Cannon, M. B. and Remington, S. J., Re-engineering redox-sensitive green fluorescent protein for improved response rate., Protein Science 15: 45-57 (2006). | PubMed |
- Chan, F. K. M., Siegel, R. M., Zacharias, D., Swofford, R., Holmes, K. L., Tsien, R. Y., and Lenardo, M. J., Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein., Cytometry 44: 361-368 (2001). | PubMed |
- Chiang, J. J. H. and Truong, K., Using co-cultures expressing fluorescence resonance energy transfer based protein biosensors to simultaneously image caspase-3 and calcium signaling., Biotechnology Letters 27: 1219-1227 (2005). | PubMed |
- Chiu, V. K., Bivona, T., Hach, A., Sajous, J. B., Silletti, J., Wiener, H., Johnson, R. L., Coxt, A. D., and Phillips, M. R., Ras signaling on the endoplasmic reticulum and the golgi., Nature Cell Biology 4: 343-350 (2002). | PubMed |
- Demaurex, N. and Frieden, M., Measurements of the free luminal ER calcium concentration with targeted "cameleon" fluorescent proteins., Cell Calcium 34: 109-119 (2003). | PubMed |
- DiPilato, L. M., Cheng, X., and Zhang, J., Fluorescent indicators of cAMP and Epac activation reveals differential dynamics of cAMP signaling within discrete subcellular compartments., Proceedings of the National Academy of Sciences (USA) 101: 16513-16518 (2004). | PubMed |
- Dooley, C. T., Dore, T. M., Hanson, G. T., Jackson, W. C., Remington, S. J., and Tsien, R. Y., Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators., Journal of Biological Chemistry 279: 22284-22293 (2004). | PubMed |
- Emmanouilidou, E., Teschemacher, A. G., Pouli, A. E., Nicholls, L. I., Seward, E. P., and Rutter, G. A., Imaging calcium concentration changes at the secretory vesicle surface with a recombinant targeted cameleon., Current Biology 9: 915-918 (1999). | PubMed |
- Erickson, M. G., Alseikhan, B. A., Peterson, B. Z., and Yue, D. T., Preassociation of calmodulin with voltage-gated calcium channels revealed by FRET in single living cells., Neuron 31: 973-985 (2001). | PubMed |
- Fan, G. Y., Fujisaki, H., Miyawaki, A., Tsay, R. K., Tsien, R. Y., and Ellisman, M. H., Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons., Biophysical Journal 76: 2412-2420 (1999). | PubMed |
- Fehr, M., Frommer, W. B., and Lalonde, S., Visualization of maltose uptake in living yeast cells by fluorescent nanosensors., Proceedings of the National Academy of Sciences (USA) 99: 9846-9851 (2002). | PubMed |
- Fehr, M., Lalonde, S., Lager, I., Wolff, M. W., and Frommer, W. B., In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors., The Journal of Biological Chemistry 278: 19127-19133 (2003). | PubMed |
- Fehr, M., Okumoto, S., Deuschle, K., Lager, I., Looger, L. L., Persson, J., Kozhuhk, L., Lalonde, S., and Frommer, W. B., Development and use of fluorescent nanosensors for metabolite imaging in living cells., Biochemical Society Transactions 33: 287-290 (2005). | PubMed |
- Fiala, A., Spall, T., Diegelmann, S., Eisermann, B., Sachse, S., Devaud, J. M., Buchner, E., and Galizia, C. G., Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons., Current Biology 12: 1877-1884 (2002). | PubMed |
- Filippin, L., Abad, M. C., Gastaldello, S., Magalhães, P. J., Sandonà, D., and Pozzan, T., Improved strategies for the delivery of GFP-based calcium sensors into the mitochondrial matrix., Cell Calcium 37: 129-136 (2005). | PubMed |
- Filippin, L., Magalhães, P. J., Di Benedetto, G., Colella, M., and Pozzan, T., Stable interactions between mitochondria and endoplasmic reticulum allow rapid accumulation of calcium in a subpopulation of mitochondria., Journal of Biological Chemistry 278: 39224-39234 (2003). | PubMed |
- Foyouzi-Youssefi, R., Arnaudeau, S., Borner, C., Kelley, W. L., Tschopp, J., Lew, D. P., Demaurex, N., and Krause, K. H., Bcl-2 decreases the free calcium concentration within the endoplasmic reticulum., Proceedings of the National Academy of Sciences (USA) 97: 5723-5728 (2000). | PubMed |
- Gaietta, G., Deerinck, T. J., Adams, S. R., Bouwer, J., Tour, O., Laird, D. W., Sosinsky, G. E., Tsien, R. Y., and Ellisman, M. H., Multicolor and electron microscopic imaging of connexin trafficking., Science 296: 503-507 (2002). | PubMed |
- Gaits, F. and Hahn, K., Shedding Light on Cell Signaling: Interpretation of FRET biosensors., Science Stke 165: 1-5 (2003). | PubMed |
- Goodwin, J. S., Drake, K. R., Remmert, C. L., and Kenworthy, A. K., Ras diffusion is sensitive to plasma membrane viscosity., Biophysical Journal 89: 1398-1410 (2005).
| PubMed | - Gorokhovatsky, A. Y., Marchenkov, V. V., Rudenko, N. V., Ivashina, T. V., Ksenzenko, V. N., Burkhardt, N., Semisotnov, G. V., Vinokurov, L. M., and Alakhov, Y. B., Fusion of Aequorea victoria GFP and aequorin provides their calcium-induced interaction that results in red shift of GFP absorption and efficient bioluminescence energy transfer., Biochemical and Biophysical Research Communications 320: 703-711 (2004). | PubMed |
- Griesbeck, O., Fluorescent proteins as sensors for cellular functions., Current Opinion in Neurobiology 14: 636-641 (2004). | PubMed |
- Griesbeck, O., Baird, G. S., Campbell, R. E., Zacharias, D. A., and Tsien, R. Y., Reducing the environmental sensitivity of yellow fluorescent protein., Journal of Biological Chemistry 276: 29188-29194 (2001). | PubMed |
- Guerrero, G. and Isacoff, E. Y., Genetically encoded optical sensors of neuronal activity and cellular function., Current Opinion in Neurobiology 11: 601-607 (2001). | PubMed |
- Guerrero, G., Siegel, M. S., Roska, B., Loots, E., and Isacoff, E. Y., Tuning FlaSh: Redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential., Biophysical Journal 83: 3607-3618 (2002). | PubMed |
- Habuchi, S., Cotlet, M., Hofkens, J., Dirix, G., Michiels, J., Vanderleyden, J., Subramaniam, V., and De Schryver, F. C., Resonance energy transfer in a calcium concentration-dependent cameleon protein., Biophysical Journal 83: 3499-3506 (2002). | PubMed |
- Han, W., Li, D., and Levitan, E. S., A new green fluorescent protein construct for localizing and quantifying peptide release., Annals New York Academy of Sciences 971: 627-633 (2002). | PubMed |
- Hanson, G. T., Aggeler, R.., Oglesbee, D., Cannon, M., Capaldi, R. A., Tsien, R. Y., and Remington, S. J., Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators., Journal of Biological Chemistry 279: 13044-13053 (2004). | PubMed |
- Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J., Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application., Biochemistry 41: 15477-15488 (2002). | PubMed |
- Harpur, A. G., Wouters, F. S., and Bastiaens, P. I. H., Imaging FRET between spectrally similar GFP molecules in single cells., Nature Biotechnology 19: 167-169 (2001).
| PubMed | - Hasan, M. T., Friedrich, R. W., Euler, T., Larkum, M. E., Giese, G., Both, M., Duebel, J., Waters, J., Bujard, H., Griesbeck, O., Tsien, R. Y., Nagai, T., Miyawaki, A., and Denk, W., Functional fluorescent calcium indicator proteins in transgenic mice under TET control., PLoS Biology 2: 0763-0775 (2004). | PubMed |
- He, L., Wu, X., Meylan, F., Olson, D. P., Simone, J., Hewgill, D., Siegel, R., and Lipsky, P. E., Monitoring caspase activity in living cells using fluorescent proteins and flow cytometry., American Journal of Pathology 164: 1901-1913 (2004). | PubMed |
- Heim, N. and Griesbeck, O., Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein., Journal of Biological Chemistry 279: 14280-14286 (2004). | PubMed |
- Heim, R., Green fluorescent protein forms for energy transfer., Methods in Enzymology 302: 408-423 (1999). | PubMed |
- Heim, R. and Tsien, R. Y., Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer., Current Biology 6: 178-182 (1996). | PubMed |
- Heinemann, U. and Hahn, M., Circular permutation of polypeptide chains: Implications for protein folding and stability., Progress in Biophysics and Molecular Biology. 64: 121-143 (1995). | PubMed |
- Higashijima, S., Masino, M. A., Mandel, G, and Fetcho, J. R., Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator., Journal of Neurophysiology 90: 3986-3997 (2003). | PubMed |
- Hoffmann, C., Gaietta, G., Bünemann, M., Adams, S. R., Oberdorff-Maass, S., Behr, B., Vilardaga, J. P., Tsien, R. Y., Ellisman, M. H., and Lohse, M. J., A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells., Nature Methods 2: 171-176 (2005). | PubMed |
- Hofmann, A., Iwai, H., Hess, S., Pluckthun, A., and Wlodawer, A., Structure of cyclized green fluorescent protein., Acta Crystallographica D58: 1400-1406 (2002). | PubMed |
- Honda, A., Adams, S. R., Sawyer, C. L., Lev-Ram, V., Tsien, R. Y., and Dostmann, W. R. G., Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator., Proceedings of the National Academy of Sciences (USA) 98: 2437-2442 (2001). | PubMed |
- Hu, C. D., Chinenov, Y., and Kerppola, T. K., Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation., Molecular Cell 9: 789-798 (2002). | PubMed |
- Hu, C. D. and Kerppola, T. K., Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis., Nature Biotechnology 21: 539-545 (2003). | PubMed |
- Ikeda, M., Sugiyama, T., Wallace, C. S., Gompf, H. S., Yoshioka, T., Miyawaki, A., and Allen, C. N., Circadian dynamics of cytosolic and nuclear calcium in single suprachiasmatic nucleus neurons., Neuron 38: 253-263 (2003). | PubMed |
- Isshiki, M., Ying, Y. S., Fujita, T., and Anderson, R. G. W., A molecular sensor detects signal transduction from caveolae in living cells., The Journal of Biological Chemistry 277: 43389-43398 (2002). | PubMed |
- Jaconi, M., Bony, C., Richards, S. M., Terzic, A., Arnaudeau, S., Vassort, G., and Pucéat, M., Inositol 1,4,5-triphosphate directs calcium flow between mitochondria and the endoplasmic/sarcoplasmic reticulum: A role in regulating cardiac autonomic calcium spiking., Molecular Biology of the Cell 11: 1845-1858 (2000). | PubMed |
- Janetopoulos, C. and Devreotes, P., Monitoring receptor-mediated activation of heterotrimeric G-proteins by fluorescence resonance energy transfer., Methods 27: 366-373 (2002). | PubMed |
- Janetopoulos, C., Jin, T., and Devreotes, P., Receptor-mediated activation of heterotrimeric G-proteins in living cells., Science 291: 2408-2411 (2001). | PubMed |
- Jayaraman, S., Haggie, P., Wachter, R. M., Remington, S. J., and Verkman, A. S., Mechanism and cellular applications of a green fluorescent protein-based halide sensor., Journal of Biological Chemistry 275: 6047-6050 (2000). | PubMed |
- Jensen, K. K., Martini, L., and Schwartz, T. W., Enhanced fluorescence resonance energy transfer between spectral variants of green fluorescent protein through zinc-site engineering., Biochemistry 40: 938-945 (2001). | PubMed |
- Ji, G., Feldman, M. E., Deng, K. Y., Greene, K. S., Wilson, J., Lee, J. C., Johnston, R. C., Rishniw, M., Tallini, Y., Zhang, J., Wier, W. G., Blaustein, M. P., Xin, H. B., Nakai, J., and Kotlikoff, M. I., Calcium-sensing transgenic mice: Post-synaptic signaling in smooth muscle.., Journal of Biological Chemistry 279: 21461-21468 (2004). | PubMed |
- Jones, J., Heim, R., Hare, E., Stack, J., and Pollok, B. A., Development and application of a GFP-FRET intracellular caspase assay for drug screening., Journal of Biomolecular Screening 5: 307-317 (2000). | PubMed |
- Jones, J. T., Myers, J. W., Ferrell, J. E., Jr., and Meyer, T., Probing the precision of the mitotic clock with a live-cell fluorescent biosensor., Nature Biotechnology 22: 306-312 (2004). | PubMed |
- Kaganman, I., DNA makes GFP shine., Nature Methods 2: 644 (2005). | PubMed |
- Kaihara, A., Kawai, Y., Sato, M., Ozawa, T., and Umezawa, Y., Locating a protein-protein interaction in living cells via Renilla luciferase complementation., Analytical Chemistry 75: 4176-4181 (2003). | Anal Chem |
- Kawai, H., Suzuki, T., Kobayashi, T., Mizuguchi, H., Hayakawa, T., and Kawanishi, T., Simultaneous imaging of initiator/effector caspase activity and mitochondrial membrane potential during cell death in living HeLa cells., Biochimica et Biophysica Acta 1693: 101-110 (2004). | PubMed |
- Kawai, H., Suzuki, T., Kobayashi, T., Sakurai, H., Ohata, H., Honda, K., Momose, K., Namekata, I., Tanaka, H., Shigenobu, K., Nakamura, R., Hayakawa, T., and Kawanishi, T., Simultaneous real-time detection of initiator- and effector-caspase activation by double fluorescence resonance energy transfer analysis., Journal of Pharmacological Sciences 97: 361-368 (2005). | PubMed |
- Kawai, Y., Sato, M., and Umezawa, Y., Single color fluorescent indicators of protein phosphorylation for multicolor imaging of intracellular signal flow dynamics., Analytical Chemistry 76: 6144-6149 (2004). | Anal Chem |
- Kenworthy, A. K., Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy., Methods 24: 289-296 (2001). | PubMed |
- Kerr, R., Lev-Ram, V., Baird, G., Vincent, P., Tsien, R. Y., and Schafer, W. R., Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans., Neuron 26: 583-594 (2000). | PubMed |
- Kim, S. B., Ozawa, T., Watanabe, S., and Umezawa, Y., High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase., Proceedings of the National Academy of Sciences (USA) 101: 11542-11547 (2004). | PubMed |
- Kneen, M., Farinas, J., Li, Y., and Verkman, A. S., Green fluorescent protein as a noninvasive intracellular pH indicator., Biophysical Journal 74: 1591-1599 (1998).
| PubMed | - Köhler, M., Zaitsev, S. V., Zaitseva, I. I., Leibiger, B., Leibiger, I. B., Turunen, M., Kapelioukh, I. L., Bakkman, L., Appelskog, I. B., de Monvel, J. B., Imreh, G., and Berggren, P. O., On-line monitoring of apoptosis in insulin-secreting cells., Diabetes 52: 2943-2950 (2003).
| PubMed | - Kraynov, V. S., Chamberlain, C., Bokoch, G. M., Schwartz, M. A., Slabaugh, S., and Hahn, K. M., Localized Rac activation dynamics visualized in living cells., Science 290: 333-337 (2000). | PubMed |
- Kuner, T. and Augustine, G. J., A genetically encoded ratiometric indicator for chloride: Capturing chloride transients in cultured hippocampal neurons., Neuron 27: 447-459 (2000). | PubMed |
- Kunkel, M. T., Ni, Q., Tsien, R. Y., Zhang, J., and Newton, A. C., Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter., The Journal of Biological Chemistry 280: 5581-5587 (2005). | PubMed |
- Kurokawa, K., Mochizuki, N., Ohba, Y., Mizuno, H., Miyawaki, A., and Matsuda, M., A pair of fluorescent resonance energy transfer-based probes for tyrosine phosphorylation of the CrkII adaptor protein in vivo., The Journal of Biological Chemistry 276: 31305-31310 (2001). | PubMed |
- Lager, I., Fehr, M., Frommer, W. B., and Lalonde, S., Development of a fluorescent nanosensor for ribose., FEBS Letters 553: 85-89 (2003). | PubMed |
- Lalonde, S., Ehrhardt, D. W., and Frommer, W. B., Shining light on signaling and metabolic networks by genetically encoded biosensors., Current Opinion in Plant Biology 8: 574-581 (2005). | PubMed |
- Larijani, B., Allen-Baume, V., Morgan, C. P., Li, M., and Cockcroft, S., EGF regulation of PITP dynamics is blocked by inhibitors of phospholipase C and of the Ras-MAP kinase pathway., Current Biology 13: 78-84 (2003). | PubMed |
- Lin, C. W., Jao, C. Y., and Ting, A. Y., Genetically encoded fluorescent reporters of histone methylation in living cells., The Journal of the American Chemical Society 126: 5982-5983 (2004). | PubMed |
- Lin, C. W. and Ting, A. Y., A genetically encoded fluorescent reporter of histone phosphorylation in living cells., Angewandte Chemie (International ed. in English) 43: 2940-2943 (2004). | PubMed |
- Lissandron, V., Terrin, A., Collini, M., D'Alfonso, L., Chirico, G., Pantano, S., and Zaccolo, M., Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor-acceptor interaction., Journal of Molecular Biology 354: 546-555 (2005).
| PubMed | - Liu, L., Yermolaieva, O., Johnson, W. A., Abboud, F. M., and Welsh, M. J., Identification and function of thermosensory neurons in Drosophila larvae., Nature Neuroscience 6: 267-273 (2003). | PubMed |
- Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G., and Tsien, R. Y., Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins., Proceedings of the National Academy of Sciences (USA) 95: 6803-6808 (1998).
| PubMed | - Luo, K. Q., Yu, V. C., Pu, Y., and Chang, D. C., Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells., Biochemical and Biophysical Research Communications 283: 1054-1060 (2001). | PubMed |
- Luo, K. Q., Yu, V. C., Pu, Y., and Chang, D. C., Measuring dynamics of caspase-8 activation in a single living HeLa cell during TNF-alpha-induced apoptosis., Biochemical and Biophysical Research Communications 304: 217-222 (2003). | PubMed |
- Magliery, T. J., Wilson, C. G. M., Pan, W., Mishler, D., Ghosh, I., Hamilton, A. D., and Regan, L., Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: Scope and mechanism., The Journal of the American Chemical Society 127: 146-157 (2005). | PubMed |
- Mahajan, N. P., Harrison-Shostak, D. C., Michaux, J., and Herman, B., Novel mutant green fluorescent protein protease substrates reveal the activation of specific caspases during apoptosis., Chemistry and Biology 6: 401-409 (1999). | PubMed |
- McAnaney, T. B., Park, E. S., Hanson, G. T., Remington, S. J., and Boxer, S. G., Green fluorescent protein variants as ratiometric dual emission pH sensors. 2. Excited-state dynamics., Biochemistry 41: 15489-15494 (2002). | PubMed |
- Metzger, F., Repunte-Canonigo, V., Matsushita, S., Akemann, W., Diez-Garcia, J., Ho, C. S., Iwasato, T., Grandes, P., Itohara, S., Joho, R. H., and Knöpfel, T., Transgenic mice expressing a pH and chlorine sensing yellow-fluorescent protein under the control of a potassium channel promoter., European Journal of Neuroscience 15: 40-50 (2002).
| PubMed | - Meyer, T. and Teruel, M. N., Fluorescence imaging of signaling networks., Trends in Cell Biology 13: 101-106 (2003). | PubMed |
- Miesenböck, G., De Angelis, D. A., and Rothman, J. E., Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins., Nature 394: 192-195 (1998). | PubMed |
- Minta, A., Kao, J. P. Y., and Tsien, R. Y., Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores., Journal of Biological Chemistry 264: 8171-8178 (1989). | PubMed |
- Mitra, R. D., Silva, C. M., and Youvan, D. C., Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein., Gene 173: 13-17 (1996). | PubMed |
- Miyawaki, A., Fluorescence imaging of physiological activity in complex systems using GFP-based probes., Current Opinion in Neurobiology 13: 591-596 (2003). | PubMed |
- Miyawaki, A., Innovations in the imaging of brain functions using fluorescent proteins., Neuron 48: 189-199 (2005). | PubMed |
- Miyawaki, A., Visualization of the spatial and temporal dynamics of intracellular signaling., Developmental Cell 4: 295-305 (2003). | PubMed |
- Miyawaki, A., Griesbeck, O., Heim, R., and Tsien, R. Y., Dynamic and quantitative calcium measurements using improved cameleons., Proceedings of the National Academy of Sciences (USA) 96: 2135-2140 (1999). | PubMed |
- Miyawaki, A., Llopis, J., Heim R., McCaffery, J. M., Adams, J. A., Ikura, M., and Tsien, R. Y., Fluorescent indicators for calcium based on green fluorescent proteins and calmodulin., Nature 388: 882-887 (1997). | PubMed |
- Miyawaki, A., Mizuno, H., Nagai, T., and Sawano, A., Development of genetically encoded fluorescent indicators for calcium., Methods in Enzymology 360: 202-225 (2003).
| PubMed | - Miyawaki, A. and Tsien, R. Y., Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein., Methods in Enzymology 327: 472-500 (2000). | PubMed |
- Mochizuki, N., Yamashita, S., Kurokawa, K., Ohba, Y., Nagai, T., Miyawaki, A., and Matsuda, M., Spatio-temporal images of growth-factor-induced activation of Ras and Rap1., Nature 411: 1065-1068 (2001). | PubMed |
- Morgan, M. J. and Thorburn, A., Measurement of caspase activity in individual cells reveals differences in the kinetics of caspase activation between cells., Cell Death and Differentiation 8: 38-43 (2001). | PubMed |
- Nagai, T. and Miyawaki, A., A high-throughput method for development of FRET-based indicators for proteolysis., Biomedical and Biophysical Research Communications 319: 72-77 (2004). | PubMed |
- Nagai, T., Sawano, A., Park, E. S., and Miyawaki, A., Circularly permuted green fluorescent proteins engineered to sense calcium., Proceedings of the National Academy of Sciences (USA) 98: 3197-3202 (2001). | PubMed |
- Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., and Miyawaki, A., Expanded dynamic range of fluorescent indicators for calcium by circularly permuted yellow fluorescent proteins., Proceedings of the National Academy of Sciences (USA) 101: 10554-10559 (2004). | PubMed |
- Nagai, Y., Miyazaki, M., Aoki, R., Zama, T., Inouye, S., Hirose, K., Iino, M., and Hagiwara, M., A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo., Nature Biotechnology 18: 313-316 (2000). | PubMed |
- Nakai, J., Ohkura, M., and Imoto, K., A high signal-to-noise calcium probe composed of a single green fluorescent protein., Nature Biotechnology 19: 137-141 (2001). | PubMed |
- Nalbant, P., Hodgson, L., Kraynov, V., Toutchkine, A., and Hahn, K. M., Activation of endogenous Cdc42 visualized in living cells., Science 305: 1615-1619 (2004).
| PubMed | - Ng, M., Roorda, R. D., Lima, S. Q., Zemelman, B. V., Morcillo, P., and Miesenböck, G., Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly., Neuron 36: 463-474 (2002). | PubMed |
- Ng, T., Squire, A., Hansra, G., Bornancin, F., Prevostel, C., Hanby, A., Harris, W., Barnes, D., Schmidt, S., Mellor, H., Bastiaens, P. I. H., and Parker, P. J., Imaging protein kinase C-alpha activation in cells., Science 283: 2085-2089 (1999). | PubMed |
- Nguyen, A. W., and Daugherty, P. S., Evolutionary optimization of fluorescent proteins for intracellular FRET., Nature Biotechnology 23: 355-360 (2005). | PubMed |
- Niethammer, P., Bastiaens, P., and Karsenti, E., Stathmin-tubulin interaction gradients in motile and mitotic cells., Science 303: 1862-1866 (2004). | PubMed |
- Nikolaev, V. O., Bünemann, M., Hein, L., Hannawacker, A., and Lohse, M. J., Novel single chain cAMP sensors for receptor-induced signal propagation., The Journal of Biological Chemistry 279: 37215-37218 (2004). | PubMed |
- Offterdinger, M., Georget, V., Girod, A., and Bastiaens, P. I. H., Imaging phosphorylation dynamics of the epidermal growth factor receptor., The Journal of Biological Chemistry 279: 36972-36981 (2004). | PubMed |
- Ohkura, M., Matsuzaki, M., Kasai, H., Imoto, K., and Nakai, J., Genetically encoded bright calcium probe applicable for dynamic calcium imaging of dendritic spines., Analytical Chemistry 77: 5861-5869 (2005). | Anal Chem |
- Oliviera, S. F., Gomez, L. L., and Dell'Acqua, M. L., Imaging kinase-AKAP79-phosphatase scaffold complexes at the plasma membrane in living cells using FRET microscopy., The Journal of Cell Biology 160: 101-112 (2003). | PubMed |
- Onuki, R., Nagasaki, A., Kawasaki, H., Baba, T., Uyeda, T. Q. P., and Taira, K., Confirmation by FRET in individual living cells of the absence of significant amyloid beta-mediated caspase 8 activation., Proceedings of the National Academy of Sciences (USA) 99: 14716-14721 (2002). | PubMed |
- Østergaard, H., Henriksen, A., Hansen, F. G., and Winther, J. R., Shedding light on disulfide bond formation: Engineering a redox switch in green fluorescent protein., The EMBO Journal 20: 5853-5862 (2001). | PubMed |
- Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., and Umezawa, Y., Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing., Analytical Chemistry 73: 2516-2521 (2001). | Anal Chem |
- Ozawa, T., Nogami, S., Sato, M., Ohya, Y., and Umezawa, Y., A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing., Analytical Chemistry 72: 5151-5157 (2000). | Anal Chem |
- Ozawa, T., Sako, Y., Sato, M., Kitamura, T., and Umezawa, Y., A genetic approach to identifying mitochondrial proteins., Nature Biotechnology 21: 287-293 (2003).
| PubMed | - Ozawa, T., Takeuchi, M., Kaihara, A., Sato, M., and Umezawa, Y., Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: Improved sensitivity and reduced screening time., Analytical Chemistry 73: 5866-5874 (2001). | Anal Chem |
- Ozawa, T. and Umezawa, Y., Detection of protein-protein interactions in vivo based on protein splicing., Current Opinion in Chemical Biology 5: 578-583 (2001). | PubMed |
- Palmer, A. E., Jin, C., Reed, J. C., and Tsien, R. Y., Bcl-2-mediated alterations in endoplasmic reticulum calcium analyzed with an improved genetically encoded fluorescent sensor., Proceedings of the National Academy of Sciences (USA) 101: 17404-17409 (2004). | PubMed |
- Pearce, L. L., Gandley, R. E., Han, W., Wasserloos, K., Stitt, M., Kanai, A. J., McLaughlin, M. K., Pitt, B. R., and Levitan, E. S., Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein., Proceedings of the National Academy of Sciences (USA) 97: 477-482 (2000). | PubMed |
- Persechini, A., Lynch, J. A., and Romoser, V. A., Novel fluorescent indicator proteins for monitoring free intracellular calcium., Cell Calcium 22: 209-216 (1997). | PubMed |
- Pertz, O. and Hahn, K. M., Designing biosensors for Rho family proteins - deciphering the dynamics of Rho family GTPase activation in living cells., Journal of Cell Science 117: 1313-1318 (2004). | PubMed |
- Phillips, M. R., Imaging signal transduction in living cells with fluorescent proteins., Science Stke 314: 1-3 (2005). | PubMed |
- Pologruto, T. A., Yasuda, R., and Svoboda, K., Monitoring neural activity and [Ca(2+)] with genetically encoded calcium indicators., The Journal of Neuroscience 24: 9572-9579 (2004). | PubMed |
- Ponsioen, B., Zhao, J., Riedl, J., Zwartkruis, F., van der Krogt, G., Zaccolo, M., Moolenaar, W. H., Bos, J. L., and Jalink, K., Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator., EMBO Reports 5: 1176-1180 (2004). | PubMed |
- Pozzan, T., Mongillo, M., and Rudolf, R., Investigating signal transduction with genetically encoded fluorescent probes., European Journal of Biochemistry 270: 2343-2352 (2003). | PubMed |
- Rehm, M., Düßmann, H., Jänicke, R. U., Tavaré, J. M., Kögel, D., and Prehn J. H. M., Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process., The Journal of Biological Chemistry 277: 24506-24514 (2002). | PubMed |
- Rieder, C. L. and Mikhailov, A., The pulse of mitosis., Nature Biotechnology 22: 279-280 (2004). | PubMed |
- Reiff, D. F., Thiel, P. R., and Schuster, C. M., Differential regulation of active zone density during long-term strengthening of Drosophila neuromuscular junctions., The Journal of Neuroscience 22: 9399-9409 (2002). | PubMed |
- Robert, V., Gurlini, P., Tosello, V., Nagai, T., Miyawaki, A., Di Lisa, F., and Pozzan, T., Beat-to-beat oscillations of mitochondrial calcium concentration in cardiac cells., EMBO Journal 20: 4998-5007 (2001). | PubMed |
- Romoser, V. A., Hinkle, P. M., and Persechini, A., Detection in living cells of calcium-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence., Journal of Biological Chemistry 272: 13270-13274 (1997). | PubMed |
- Rudolf, R., Mongillo, M., Rizzuto, R., and Pozzan, T., Looking forward to seeing calcium., Nature Reviews Molecular Cell Biology 4: 579-586 (2003). | PubMed |
- Sakai, R., Repunte-Canonigo, V., Raj, C. D., and Knöpfel, T., Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein., European Journal of Neuroscience 13: 2314-2318 (2001). | PubMed |
- Sankaranarayanan, S., De Angelis, D., Rothman, J. E., and Ryan, T. A., The use of pHluorins for optical measurements of presynaptic activity., Biophysical Journal 79: 2199-2208 (2000). | PubMed |
- Sasaki, K., Sato, M., and Umezawa, Y., Fluorescent indicators for Akt/protein kinase B and dynamics of Akt activity visualized in living cells., The Journal of Biological Chemistry 278: 30945-30951 (2003). | PubMed |
- Sato, M., Hida, N., Ozawa, T., and Umezawa, Y., Fluorescent indicators for cyclic GMP based on cyclic GMP-dependent protein kinase I-alpha and green fluorescent proteins., Analytical Chemistry 72: 5918-5924 (2000). | Anal Chem |
- Sato, M., Ozawa, T., Inukai, K., Asano, T., and Umezawa, Y., Fluorescent indicators for imaging protein phosphorylation in single living cells., Nature Biotechnology 20: 287-294 (2002). | PubMed |
- Sato, M., Ueda, Y., Takagi, T., and Umezawa, Y., Production of PtdInsP3 at endomembranes is triggered by receptor endocytosis., Nature Cell Biology 5: 1016-1022 (2003). | PubMed |
- Sato, M. and Umezawa, Y., Imaging protein phosphorylation by fluorescence in single living cells., Methods 32: 451-455 (2004). | PubMed |
- Schleifenbaum, A., Stier, G., Gasch, A., Sattler, M., and Schultz, C., Genetically encoded FRET probe for PKC activity based on pleckstrin., The Journal of the American Chemical Society 126: 11786-11787 (2004). | PubMed |
- Schultz, C., Schleifenbaum, A., Goedhart, J., and Gadella, T. W. J., Jr., Multiparameter imaging for the analysis of intracellular signaling., ChemBioChem: a European Journal of Chemical Biology 6: 1323-1330 (2005). | PubMed |
- Shimozono, S., Fukano, T., Kimura, K. D., Mori, I., Kirino, Y., and Miyawaki, A., Slow calcium dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping., EMBO Reports 5: 521-526 (2004). | PubMed |
- Shimozono, S., Fukano, T., Nagai, T., Kirino, Y., Mizuno, H., and Miyawaki, A., Confocal imaging of subcellular calcium concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein., Science's STKE 125: PL4 (2002).
| PubMed | - Shyu, Y. J., Liu, H., Deng, X., and Hu, C. D., Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions., Biotechniques 40: 61-66 (2006). | Biotechniques |
- Siegel, M. S. and Isacoff, E. Y., A genetically encoded optical probe of membrane voltage., Neuron 19: 735-741 (1997). | PubMed |
- Siegel, R. M., Chan, F. K. M., Zacharias, D. A., Swofford, R., Holmes, K. L., Tsien, R. Y., and Lenardo, M. J., Measurement of molecular interactions in living cells by fluorescence resonance energy transfer between variants of green fluorescent protein., Science STKE 38: 1-6 (2000). | PubMed |
- Siegel, R. M., Frederiksen, J. K., Zacharias, D. A., Chan, F. K. M., Johnson, M., Lynch, D., Tsien, R. Y., and Lenardo, M. J., Fas preassociation required for apoptosis signaling and dominant inhibition by pathogenic mutations., Science 288: 2354-2357 (2000).
| PubMed | - Smallridge, R., A changed cameleon., Nature Reviews Molecular Cell Biology 6: 92-93 (2005). | Nat Rev Mol Cell Biol |
- Stains, C. I., Porter, J. R., Ooi, A. T., Segal, D. J., and Ghosh, I., DNA sequence-enabled reassembly of the green fluorescent protein., Journal of the American Chemical Society 127: 10782-10783 (2005). | PubMed |
- Suzuki, H., Kerr, R., Bianchi, L., Frøkjær-Jensen, C., Slone, D., Xue, J., Gerstbrein, B., Driscoll, M., and Schafer, W. R., In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation., Neuron 39: 1005-1017 (2003). | PubMed |
- Suzuki, M., Ito, Y., Sakata, I., Sakai, T., Husimi, Y., and Douglas, K. T., Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein., Biochemical and Biophysical Research Communications 330: 454-460 (2005). | PubMed |
- Takemoto, K., Nagai, T., Miyawaki, A., and Miura, M., Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects., Journal of Cell Biology 160: 235-243 (2003). | PubMed |
- Tanimura, A., Nezu, A., Morita, T., Turner, R. J., and Tojyo, Y., Fluorescent biosensor for quantitative real-time measurements of inositol 1,4,5-triphosphate in single living cells., The Journal of Biological Chemistry 279: 38095-38098 (2004). | PubMed |
- Tawa, P., Tam, J., Cassady, R., Nicholson, D. W., and Xanthoudakis, S., Quantitative analysis of fluorescent caspase substrate cleavage in intact cells and identification of novel inhibitors of apoptosis., Cell Death and Differentiation 8: 30-37 (2001).
| PubMed | - Ting, A. Y., Kain, K. H., Klemke, R. L., and Tsien, R. Y., Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells., Proceedings of the National Academy of Sciences (USA) 98: 15003-15008 (2001). | PubMed |
- Topell, S., Hennecke, J., and Glockshuber, R., Circularly permuted variants of the green fluorescent protein., FEBS Letters 457: 283-289 (1999). | PubMed |
- Truong, K., Sawano, A., Mizuno, H., Hama, H., Tong, K. I., Mal, T. K., Miyawaki, A., and Ikura, M., FRET-based in vivo calcium imaging by a new calmodulin-GFP fusion molecule., Nature Structural Biology 8: 1069-1073 (2001). | PubMed |
- Tsien, R. Y. and Miyawaki, A., Seeing the machinery of live cells., Science 280: 1954-1955 (1998). | PubMed |
- Tyas, L., Brophy, V. A., Pope, A., Rivett, A. J., and Tavaré, J. M., Rapid caspase-3 activation during apoptosis revealed using fluorescence-resonance energy transfer., EMBO Reports 1: 266-270 (2000). | PubMed |
- Umezawa, Y., Genetically encoded optical probes for imaging cellular signaling pathways., Biosensors and Bioelectronics 20: 2504-2511 (2005). | PubMed |
- van der Wal, J., Habets, R., Várnai, P., Balla, T., and Jalink, K., Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer., The Journal of Biological Chemistry 276: 15337-15344 (2001). | PubMed |
- Vanderklish, P. W., Krushel, L. A., Holst, B. H., Gally, J. A., Crossin, K. L., and Edelman, G. M., Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer., Proceedings of the National Academy of Sciences (USA) 97: 2253-2258 (2000). | PubMed |
- Verveer, P. J., Wouters, F. S., Reynolds, A. R., and Bastiaens, P. I. H., Quantitative imaging of lateral ErbB1 receptor signal propagation in the plasma membrane., Science 290: 1567-1570 (2000). | PubMed |
- Vilardaga, J. P., Bünemann, M., Krasel, C., Castro, M., and Lohse, M. J., Measurement of the millisecond activation switch of G protein-coupled receptors in living cells., Nature Biotechnology 21: 807-812 (2003). | PubMed |
- Violin, J. D., Zhang, J., Tsien, R. Y., and Newton, A. C., A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C., The Journal of Cell Biology 161: 899-909 (2003). | PubMed |
- Wachter, R. M. and Remington, S. J., Sensitivity of the yellow variant of green fluorescent protein to halides and nitrate., Current Biology 9: R628-R629 (1999).
| PubMed | - Wang, F., Chen, T. S., Xing, D., Wang, J. J., and Wu, Y. X., Measuring dynamics of caspase-3 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation., Lasers in Surgery and Medicine 36: 2-7 (2005). | PubMed |
- Wang, J. W., Wong, A. M., Flores, J., Vosshall, L. B., and Axel, R., Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain., Cell 112: 271-282 (2003). | PubMed |
- Wang, Y., Botvinick, E. L., Zhao, Y., Berns, M. W., Usami, S., Tsien, R. Y., and Chien, S., Visualizing the mechanical activation of Src., Nature 434: 1040-1045 (2005). | PubMed |
- Weijer, C. J., Visualizing signals moving in cells., Science 300: 96-100 (2003). | PubMed |
- Xu, X., Gerard, A. L. V., Huang, B. C. B., Anderson, D. C., Payan, D. G., and Luo, Y., Detection of programmed cell death using fluorescence energy transfer., Nucleic Acids Research 26: 2034-2035 (1998). | PubMed |
- Ye, K. and Schultz, J. S., Genetic engineering of an allosterically based glucose indicator protein for continuous glucose monitoring by fluorescence resonance energy transfer., Analytical Chemistry 75: 3451-3459 (2003). | Anal Chem |
- Yoshizaki, H., Ohba, Y., Kurokawa, K., Itoh, R. E., Nakamura, T., Mochizuki, N., Nagashima, K., and Matsuda, M., Activity of Rho-family GTPases during cell division as visualized with FRET-based probes., The Journal of Cell Biology 162: 223-232 (2003). | PubMed |
- Yu, D., Baird, G. S., Tsien, R. Y., and Davis, R. L., Detection of calcium transients in Drosophila mushroom body neurons with camgaroo reporters., Journal of Neuroscience 23: 64-72 (2003). | PubMed |
- Yu, R. and Hinkle, P. M., Rapid turnover of calcium in the endoplasmic reticulum during signaling., The Journal of Biological Chemistry 275: 23648-23653 (2000). | PubMed |
- Zaccolo, M., Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses., Circulation Research 94: 866-873 (2004).
| PubMed | - Zaccolo, M., Cesetti, T., Di Benedetto, G., Mongillo, M., Lissandron, V., Terrin, A., and Zamparo, I., Imaging the cAMP-dependent signal transduction pathway(1)., Biochemical Society Transactions 33: 1323-1326 (2005). | PubMed |
- Zaccolo, M., De Giorgi, F., Cho, C. Y., Feng, L., Knapp, T., Negulescu, P. A., Taylor, S. S., Tsien, R. Y., and Pozzan, T., A genetically encoded, fluorescent indicator for cyclic AMP in living cells., Nature Cell Biology 2: 25-29 (2000). | PubMed |
- Zaccolo, M., Magalhães, P., and Pozzan, T., Compartmentalisation of cAMP and calcium signals., Current Opinion in Cell Biology 14: 160-166 (2002). | PubMed |
- Zaccolo, M. and Pozzan, T., Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes., Science 295: 1711-1715 (2002). | PubMed |
- Zaccolo, M. and Pozzan, T., Imaging signal transduction in living cells with GFP-based probes., IUBMB Life 49: 375-379 (2000). | PubMed |
- Zacharias, D. A., Baird, G., S., and Tsien, R. Y., Recent advances in technology for measuring and manipulating cell signals., Current Opinion in Neurobiology 10: 416-421 (2000). | PubMed |
- Zhang, J., Ma, Y., Taylor, S. S., and Tsien, R. Y., Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering., Proceedings of the National Academy of Sciences (USA) 98: 14997-15002 (2001). | PubMed |
- Zhang, S., Ma, C., and Chalfie, M., Combinatorial marking of cells and organelles with reconstituted fluorescent proteins., Cell 119: 137-144 (2004). | PubMed |
- Zhang, Y., Haskins, C., Lopez-Cruzan, M., Zhang, J., Centonze, V. E., and Herman, B., Detection of mitochondrial caspase activity in real time in situ in live cells., Microscopy and Microanalysis 10: 442-448 (2004). | PubMed |