Solutions en sciences de la vie
Accueil/ / Centre de ressources en microscopie/ Introduction à la microscopie/ Techniques de microscopie spécialisées/ Fluorescence/ Digital Image Gallery - Fluorescence Microscopy/ Fluorescence Digital Image Gallery - Cells in Culture/ cef/ Transformed Chicken Embryo Fibroblast Cells (UMNSAH/DF-1 Line)
Transformed Chicken Embryo Fibroblast Cells (UMNSAH/DF-1 Line)
In a double immunofluorescence experiment, a log phase adherent monolayer culture of chicken embryo fibroblast cells was fixed, permeabilized, blocked with 10 percent normal goat serum, and treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Texas Red and Alexa Fluor 488, respectively. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin.
Sorry, this page is not
available in your country.