Applications

For Cell Biology Research

Cell biology requires a high-sensitivity imaging system to minimize phototoxicity to live organisms such as zebrafish and C. elegans. Imaging large pieces of tissue and small organisms may also require both high speeds as well as large fields of view to see the entire organism in context, requiring precise automation and excellent optics. The FV3000 system is designed to image large tissues and small organisms with accurate stage control, image stitching, and an optical design that facilitates very low to high magnification (1.25X up to 150X). To facilitate the removal of autofluorescence from the detection signal, the fully-spectral system comes equipped with advanced spectral unmixing software, enabling separation of autofluorescence and overlapping spectra (e.g. GFP/YFP).

Blood flow imaging and velocity measurements by cellSens software kymograph analysis. Image data courtesy of: Dr.Takuya Hiratsuka, Dr. Michiyuki Matsuda, Graduate school of Biostudies, Kyoto University.	For Cancer Research The FV3000 series incorporates the technologies necessary for powerful cancer research imaging studies. In live cell cancer studies, sensitive fluorescence detection, optimized optics, and analytical tools such as cell counting and segmentation analysis are essential. With the emergence of microfluidics and a focus on circulating tumor cells, high-speed acquisitions can make the difference between success and failure in an experiment. Accuracy and repeatability are equally important; cell cycle checkpoint times must be reliably tracked, 3D images of cells must correctly represent their shape and size, and images need to be bright and clear for segmentation analysis. Our silicone objectives are optimized for 3D tissue imaging by closely matching the refractive index of living tissue, enabling more accurate volume renderings. When coupled with the high-sensitivity cooled GaAsP detection unit, bright images with high signal-to-noise can easily be achieved for reliably accurate and reproducible results. In cancer research, it’s important to know that apoptosis is part of the experiment and not being caused by phototoxicity. The high sensitivity of the FV3000 coupled with the laser power monitor and freely selectable laser power down to 0.01% help minimize phototoxicity for more physiologically accurate imaging data. The system’s spectral sensitivity and accuracy enable researchers to conduct multicolor fluorescence labeling experiments with multiple biomarkers without fear of stressing cells from too much laser exposure.

For Cancer Research

Blood flow imaging and velocity measurements by cellSens software kymograph analysis.
Image data courtesy of: Dr.Takuya Hiratsuka, Dr. Michiyuki Matsuda, Graduate school of Biostudies, Kyoto University.

For Cancer Research

The FV3000 series incorporates the technologies necessary for powerful cancer research imaging studies. In live cell cancer studies, sensitive fluorescence detection, optimized optics, and analytical tools such as cell counting and segmentation analysis are essential. With the emergence of microfluidics and a focus on circulating tumor cells, high-speed acquisitions can make the difference between success and failure in an experiment.
Accuracy and repeatability are equally important; cell cycle checkpoint times must be reliably tracked, 3D images of cells must correctly represent their shape and size, and images need to be bright and clear for segmentation analysis. Our silicone objectives are optimized for 3D tissue imaging by closely matching the refractive index of living tissue, enabling more accurate volume renderings. When coupled with the high-sensitivity cooled GaAsP detection unit, bright images with high signal-to-noise can easily be achieved for reliably accurate and reproducible results.
In cancer research, it’s important to know that apoptosis is part of the experiment and not being caused by phototoxicity. The high sensitivity of the FV3000 coupled with the laser power monitor and freely selectable laser power down to 0.01% help minimize phototoxicity for more physiologically accurate imaging data. The system’s spectral sensitivity and accuracy enable researchers to conduct multicolor fluorescence labeling experiments with multiple biomarkers without fear of stressing cells from too much laser exposure.

For Stem Cell Research Stem cell imaging requires increased levels of automation and long-term time-lapse capabilities. The FV3000 system is designed to image cells over multiple days with accurate timing, low phototoxicity, and accurate focus. To maintain focus during multipoint time-lapse imaging in microplates, the system can be enhanced with a Z-drift compensator (IX3-ZDC2). For long experiments, add the laser power monitor to maintain consistent laser exposure for excellent laser stability. Users performing stem cell imaging benefit from high-sensitivity detection, silicone objectives, low phototoxicity from the resonant scanner, and the higher throughput from high-speed scanning. Precise stimulation control means photoconversion is simple and efficient, so cells can be reliably stimulated and imaged over multiple days for cell lineage tracking. Whether stem cell cultures are in microplates, single dishes, or microfluidic devices, the FV3000 software and automation makes workflows simple. The stage navigator includes well plate navigation and makes it easy to save, modify, and re-load frequently used plate settings and acquisition conditions. Users can quickly image individual lanes of microfluidic channels. The sequence manager makes it easy to set up long-term time-lapse imaging. Users can adjust the speed and timing of acquisitions while maintaining accurate timing. Quickly visualize and download publication- and presentation-ready 3D and 4D image data with the intuitive rendering software included with the FV3000 software suite. Once imaging is completed, the macro functionality in cellSens analysis facilitates 2D cell counting and segmentation with a single mouse click.	MatTek EpiDermFT Tissue Model: Immunofluorescence labeled with 6 targets of interest. 1. Abcam DRAQ5 ab108410, 2. Abcam Anti-GAPDH (Alexa Fluor 405) ab206372, 3. Abcam Anti-Tubulin (Alexa Fluor 488) ab1955883, 4. Abcam Anti-Fibrillarin (Alexa Fluor 568) ab202540, 5. Abcam Anti-Vimentin (Alexa Fluor 594) ab154207, 6. Abcam Anti-Ki67 (Alexa Fluor 647) ab 194724. Sample courtesy MatTek.

For Stem Cell Research

Stem cell imaging requires increased levels of automation and long-term time-lapse capabilities. The FV3000 system is designed to image cells over multiple days with accurate timing, low phototoxicity, and accurate focus. To maintain focus during multipoint time-lapse imaging in microplates, the system can be enhanced with a Z-drift compensator (IX3-ZDC2). For long experiments, add the laser power monitor to maintain consistent laser exposure for excellent laser stability.

Users performing stem cell imaging benefit from high-sensitivity detection, silicone objectives, low phototoxicity from the resonant scanner, and the higher throughput from high-speed scanning. Precise stimulation control means photoconversion is simple and efficient, so cells can be reliably stimulated and imaged over multiple days for cell lineage tracking. Whether stem cell cultures are in microplates, single dishes, or microfluidic devices, the FV3000 software and automation makes workflows simple. The stage navigator includes well plate navigation and makes it easy to save, modify, and re-load frequently used plate settings and acquisition conditions. Users can quickly image individual lanes of microfluidic channels. The sequence manager makes it easy to set up long-term time-lapse imaging. Users can adjust the speed and timing of acquisitions while maintaining accurate timing. Quickly visualize and download publication- and presentation-ready 3D and 4D image data with the intuitive rendering software included with the FV3000 software suite. Once imaging is completed, the macro functionality in cellSens analysis facilitates 2D cell counting and segmentation with a single mouse click.

For Stem Cell Research

MatTek EpiDermFT Tissue Model: Immunofluorescence labeled with 6 targets of interest. 1. Abcam DRAQ5 ab108410, 2. Abcam Anti-GAPDH (Alexa Fluor 405) ab206372, 3. Abcam Anti-Tubulin (Alexa Fluor 488) ab1955883, 4. Abcam Anti-Fibrillarin (Alexa Fluor 568) ab202540, 5. Abcam Anti-Vimentin (Alexa Fluor 594) ab154207, 6. Abcam Anti-Ki67 (Alexa Fluor 647) ab 194724. Sample courtesy MatTek.

	For Neuroscience Research The FV3000 confocal laser scanning microscope is compatible with a variety of objectives from 1.25x to 150x, enabling both macro overviews as well as high-resolution image acquisition. Obtain images of whole brain tissue sections to get easily identify areas of interest, and then switch to a high magnification objective to acquire high-resolution images of target neurons. You can even observe the fine structure of dendritic spines using Olympus super resolution technology. With the system’s TruSpectal detection technology, it’s possible to image neurons labeled with multiple colors and clearly separate the individual spectra. FV3000 Upright System — Electrophysiology Configuration This configuration offers a larger working space, so there’s more room around the objective for electrophysiology equipment. You can add additional space by lowering the height of the stage to accommodate experiments that involve large samples. Fast phenomena, such as calcium ion dynamics related to neuronal signal transmission, can be captured at up to 438 frames per second with the available resonant scanner. With the TTL signal I/O interface box, you can synchronize image acquisition with electrophysiology experiment. Additionally, you can use the remote control software development kit to control both your FV3000 microscope and your electrophysiology equipment from a single software platform. Click here for the details on upright microscope frame

For Neuroscience Research

The FV3000 confocal laser scanning microscope is compatible with a variety of objectives from 1.25x to 150x, enabling both macro overviews as well as high-resolution image acquisition. Obtain images of whole brain tissue sections to get easily identify areas of interest, and then switch to a high magnification objective to acquire high-resolution images of target neurons. You can even observe the fine structure of dendritic spines using Olympus super resolution technology. With the system’s TruSpectal detection technology, it’s possible to image neurons labeled with multiple colors and clearly separate the individual spectra.

FV3000 Upright System — Electrophysiology Configuration

This configuration offers a larger working space, so there’s more room around the objective for electrophysiology equipment. You can add additional space by lowering the height of the stage to accommodate experiments that involve large samples. Fast phenomena, such as calcium ion dynamics related to neuronal signal transmission, can be captured at up to 438 frames per second with the available resonant scanner.
With the TTL signal I/O interface box, you can synchronize image acquisition with electrophysiology experiment. Additionally, you can use the remote control software development kit to control both your FV3000 microscope and your electrophysiology equipment from a single software platform.

Click here for the details on upright microscope frame

For Advanced Applications

Both the FV3000 and FV3000RS microscopes have a range of standard and optional advanced application features, including Olympus Super Resolution (FV-OSR), photostimulation, spectral unmixing, and an external beam combiner. With precise laser control and our patented super resolution method, the FV3000 can acquire images with a resolution down to 120 nm, similar to structured illumination methods. Spectral unmixing is robust for a range of applications, while photoconversion and photostimulation are efficient and precise, enabling high-speed targeted path scanning and stimulation mapping studies.

The Sequence Manager makes it easy to reliably achieve complex cell cycle imaging protocols. Advanced applications, such as random access or targeted path scanning, enable high signal-to-noise multipoint fluorescence measurements for in vitro neuronal cell signaling studies, while real-time processing and triggering help provide accurate and coordinated timing control for TTL-driven perfusion devices, stimulators, or other 3rd party peripherals. Macro-to-micro functionality is easy thanks to the stage navigator, automation built into the IX83 microscope, and the ability to save and reload software layouts, workflows, and experiment conditions.

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FV3000

Applications

For Cell Biology Research

For Cancer Research

For Stem Cell Research

For Neuroscience Research

FV3000 Upright System — Electrophysiology Configuration

For Advanced Applications

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