Cancer Research and Multiplexing

Cancer research involves the analysis of carcinogenesis, including proliferation, invasion, and metastasis, and the advancement of cancer diagnostics and therapies. For detailed tumor classification, fluorescence multiplexing is a powerful tool, which uses a large number of different tumor markers and fluorophores. Putting X Line lenses at the heart of your imaging system delivers unmatched flexibility in fluorophore selection and provides rich, accurate data.

Excellent Multiplexing

X Line objective’s broad chromatic aberration correction from 400–1000 nm helps you accurately analyze colocalization of a wide range of fluorophores. Multichannel acquisition is offered from slide scanning to laser scanning microscopes via various Olympus imaging systems, and our X Line objectives are always the lenses of choice for these systems.

Lung tissue imaged on a VS200 with a  20X X Line objective stained with an Ultivue PD-L1 kit multiplex kit; Dapi: Nuclear Counterstain, FITC: CD8, TRITC: CD68, Cy5: PD-L1, Cy7: panCK.

Image data courtesy of Ultivue Inc.

Bright Imaging of Thick Tissue

A Line silicone immersion objective lenses compensate for both spherical and chromatic aberrations, and they have high transmission from the visible to the near-infrared region. The refractive index of silicone oil (ne≈1.40) is close to that of living tissue (ne≈1.38), enabling high-resolution observations deep inside living tissue, with minimal spherical aberration and accurate 3D morphology.

Mouse mPFC labeled with glial fibrillary acidic protein (GFAP; astrocyte marker; yellow), calmodulin-dependent protein kinase II (CaMKII; pyramidal neuron marker; red), amphoterin-induced protein 1 precursor (AMIGO-1; neuronal membrane marker; cyan), parvalbumin (PV; inhibitory neuron marker; purple), ankyrin-G (AnkG; axon initial segment marker; green), and nuclear yellow (nuclei marker; blue). (Left) Individual channels of the six fluorophores. (Right) Overlay image.