Live Cell Imaging Microscopy to Study Cell Polarity Establishment

Proper establishment of cell polarity is critical for stem cell lineage and development. Any error in the polarity establishment can lead to defects during development and self-renewal of stem cells.

In the one-cell stage of the C. elegans embryonic centrosome, the localized signal is critical for regulating polarity establishment, yet the key component for this process was unknown until now.

In this webinar, we shall discuss our new finding of the role of the evolutionarily conserved Aurora A kinase in accurate polarity establishment via the modulation of actomyosin-based contractility through RhoGEF ECT2.

We will also discuss how the Olympus FV3000 confocal microscope was optimized for fixed and live cell imaging and used effectively for our experiments.

Presenters:

Dr. Sachin Kotak Assistant Professor, Indian Institute of Science (IISc), Bangalore Dr. Sachin Kotak holds a PhD in Molecular Cell Biology from Johann Wolfgang Goethe University, Germany. He completed his post-doctoral work in Cell Biology from the Swiss Institute of Experimental Cancer Research-EPFL, Switzerland. He is currently an Assistant Professor at the Department of Microbiology and Cell Biology at the Indian Institute of Sciences, Bangalore, India. His lab studies the mechanisms of spindle orientation and polarity establishment in animal cells.

Live Cell Imaging Microscopy to Study Cell Polarity Establishment Proper establishment of cell polarity is critical for stem cell lineage and development. Any error in the polarity establishment can lead to defects during development and self-renewal of stem cells. In the one-cell stage of the C. elegans embryonic centrosome, the localized signal is critical for regulating polarity establishment, yet the key component for this process was unknown until now. In this webinar, we shall discuss our new finding of the role of the evolutionarily conserved Aurora A kinase in accurate polarity establishment via the modulation of actomyosin-based contractility through RhoGEF ECT2. We will also discuss how the Olympus FV3000 confocal microscope was optimized for fixed and live cell imaging and used effectively for our experiments. Presenters: Dr. Sachin Kotak Assistant Professor, Indian Institute of Science (IISc), Bangalore Dr. Sachin Kotak holds a PhD in Molecular Cell Biology from Johann Wolfgang Goethe University, Germany. He completed his post-doctoral work in Cell Biology from the Swiss Institute of Experimental Cancer Research-EPFL, Switzerland. He is currently an Assistant Professor at the Department of Microbiology and Cell Biology at the Indian Institute of Sciences, Bangalore, India. His lab studies the mechanisms of spindle orientation and polarity establishment in animal cells. Complete el formulario para acceder al seminario web. EscogerAfghanistanAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBosnia and HerzegovinaBotswanaBrazilBritish Virgin IslandsBrunei DarussalamBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos IslandsColombiaComorosCongoCongo, Democratic Republic of the Cook IslandsCosta RicaCote d'IvoireCroatiaCubaCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland Islands (Malvinas)Faroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and Mcdonald IslandsHoly SeeHondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Dem. People's Rep. of Korea, Republic of KuwaitKyrgyzstanLao People's Dem. Rep.LatviaLebanonLesothoLiberiaLibyan Arab JamahiriyaLiechtensteinLithuaniaLuxembourgMacaoMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesia, Federated States ofMoldova, Republic of MonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNetherlands AntillesNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorthern Mariana IslandsNorwayOmanPakistanPalauPalestinePanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarReunionRomaniaRussian FederationRwandaSaint BarthelemySaint HelenaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSpainSri LankaSudanSurinameSvalbard and Jan Mayen IslandsSwazilandSwedenSwitzerlandSyrian Arab RepublicTaiwanTajikistanTanzania, United Republic of ThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluU.S. Virgin IslandsUSAUgandaUkraineUnited Arab EmiratesUnited KingdomUruguayUzbekistanVanuatuVenezuelaViet NamWallis and Futuna IslandsWestern SaharaYemenZambiaZimbabwe Ubicación * Nombre de la compañía * Nombre * Apellido * Correo electrónico * Ciudad Código postal * Suscríbase al boletín de noticias de Evident Scientific Please choose division Investigación académicaBiotecnologíaClínicoEducaciónDescubrimiento de fármacosMedicina regenerativaComponentes OEMOtros Reconozco estar de acuerdo con las Condiciones de Uso, y confirmo haber leído y comprendido la Política de privacidad* Enviar If you already have your personal ID enter it here: