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A culture of Indian Muntjac deer skin fibroblast cells was transfected with a pEYFP-Mitochondria (enhanced yellow fluorescent protein) chimeric plasmid subcellular localization vector. After fixation and permeabilization, the cells were labeled with Alexa Fluor 568 conjugated to phalloidin and DAPI, which target the F-actin cytoskeletal network and DNA in the cell nucleus, respectively.
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