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MitoTracker, Actin, and DNA Probes with Indian Muntjac Deer Skin Fibroblast Cells
A triple fluorophore combination of MitoTracker Orange CMTMRos, BODIPY FL conjugated to phalloidin, and TO-PRO-3 was used to label an adherent log phase culture of Indian Muntjac cells for mitochondria, the filamentous actin network, and nuclear DNA, respectively. The cells were first treated with the MitoTracker probe in growth medium for one hour, washed and fixed with paraformaldehyde (prepared in growth medium), permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with the conjugated phalloidin and counterstained with the cyanine monomer (TO-PRO-3) reagent.
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