Two Multiphoton Microscopes Sharing One IR Laser

This system allows two microscopes to share a single laser and provides cutting-edge technology for various areas of scientific research such as neuroscience and cell biology. In this way, the FV1200MPE system types M, B, S and T can be combined on a single platform.

New High Reflection Silver Coated Scanning Mirrors

Anti-oxidization silver coating ensures durability and improves reflection efficiency in both excitation and emission with the set of two galvanometer scanning mirrors, increasing the reflectance in the visible range by 5 - 15 %, and IR reflectance up to 22 %, improving multiphoton excitation efficiency by nearly 50 % at depth.

Motorized Keplerian Beam Expander

As the probability for multiphoton excitation is very low, it is crucial to have a very small excitation volume (requiring objectives with high NA) and to guide as much IR light to the sample as possible. This means the rear opening (backfocal aperture) of the objective should be filled completely by the excitation laser beam. Having the beam larger than the backfocal aperture would mean loss of excitation laser light. The backfocal apertures of objectives sometimes differ by more than a factor of two, leading either to losses or to reduced NA. By implementing motorized beam expander optics, Olympus ensures that for all the objectives and different wavelengths used, the size of the IR beam is right for the backfocal aperture of the objective.

Ultra-fast and Flexible Laser Control

The integrated AOM module enables high-speed control of the laser, both for switching on/off and for adjusting intensity.

Femtosecond IR Laser with Pulse Pre-compensation

In multiphoton microscopy, a pulsed laser is required to achieve sufficient excitation efficiency of the fluorophores. As laser pulses (typically ~ 100 fs) are stretched when travelling through microscope optics (dispersion), fluorophore excitation and fluorescence detection will become less efficient. By using a femtosecond-pulsed laser with pulse pre-compensation (negative chirp), this effect can be strongly reduced and the original pulse-width almost completely restored. For specifications please see the FV1000MPE M, B, S, and T-System component pages.