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Challenges Solutions of Confocal Microscopy in Live Cell Imaging

Duration: 30 minutes

Live cell imaging is a powerful tool to visualize dynamic processes in cells and tissues, ultimately showing changes happening in their biological environment. The need for higher resolution, 3D information, and better contrast often leads to the usage of confocal microscopy.
However, there are different strategies to overcome these effects, including the use of more sensitive detectors or reducing the excitation time per pixel to avoid dark states of fluorophores.
In this shortcast we will discuss the image formation on spinning disk and point scanning confocals and compare them across various applications.

Presenter:

Florian Eich
Senior Business Development Manager Life Science
Olympus Life Science

Challenges Solutions of Confocal Microscopy in Live Cell Imaging

Challenges Solutions of Confocal Microscopy in Live Cell Imaging

Duration: 30 minutes

Live cell imaging is a powerful tool to visualize dynamic processes in cells and tissues, ultimately showing changes happening in their biological environment. The need for higher resolution, 3D information, and better contrast often leads to the usage of confocal microscopy.
However, there are different strategies to overcome these effects, including the use of more sensitive detectors or reducing the excitation time per pixel to avoid dark states of fluorophores.
In this shortcast we will discuss the image formation on spinning disk and point scanning confocals and compare them across various applications.

Presenter:

Florian Eich
Senior Business Development Manager Life Science
Olympus Life Science

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Related Products
Confocal Imaging Microscope System

IXplore Spin

The IXplore Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.

  • Real-time controller (U-RTCE) helps optimize the device’s speed and precision during automated acquisition
  • TruFocus™ Z-drift compensation system maintains focus for each frame
  • Precise 3D imaging with improved light collection using X Line™ objectives
  • Upgrade to the IXplore SpinSR super resolution system as your research progresses
Super Resolution Microscope System

IXplore IX83 SpinSR

The IXplore SpinSR system is our confocal super resolution microscope optimized for 3D imaging of live cell specimens. Like the IXplore Spin system, it has a spinning disk system for fast 3D imaging while limiting phototoxicity and bleaching. However, it achieves super resolution images down to 120 nm XY and enables you to switch between widefield, confocal, and super resolution with the click of a button.

  • Sharp, clear super resolution imaging down to 120 nm XY, owing to Olympus Super Resolution (OSR)
  • Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
  • Use two cameras simultaneously to achieve fast, two-color super-resolution imaging
  • Super resolution imaging with the world’s first plan apochromat objectives with a numerical aperture (NA) of 1.5*
* As of November 2018. According to Olympus research.
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