Low Autofluorescence Immersion Oil

Low autofluorescence immersion oil is ideal for fluorescence microscopy since it reduces the autofluorescence normally associated with standard immersion oils. Reduced noise (autofluorescence) results in an increased SNR for better fluorescent imaging. Temporal change of autofluorescence is also reduced. This is especially useful for quantitative observations of single molecule fluorescence, which can be easily affected by noise. Resistance to crystallization enables the immersion oil to be used over long periods of time. (Durability testing guarantees usability for 14 days at 40 °C or 104 °F). The refraction index is the same as other Olympus products for easy integration with your existing microscopy systems.

The Effect of Low Autofluorescence Oil

Using low autofluorescence oil improves image contrast in fluorescence microscopy applications. See the difference in the images below, which show fluorescent imaging of bovine pulmonary artery endothelial cells using low autofluorescence oil and standard immersion oil.