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Overview
 | The Olympus IX83 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs. |
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Expandable to Meet Growing Research NeedsThe fully-motorized IX83 is designed to satisfy a variety of research needs. With additional modules providing expanded functionality, both microscope options enable a multitude of imaging techniques, ranging from casual documentation to long-term time-lapse imaging and other demanding techniques. |
IX83: Two-deck System
Enables high-speed, fully automated device selection during live cell research and advanced image acquisition. The two-deck configuration provides excellent expandability. | IX83: One-deck System
An intelligent, motorized microscope featuring a large field number (FN22, left side port) and TruFocus compatibility, thus creating a new standard for live cell imaging. |
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Specifications
| Observation Method > Fluorescence (Blue/Green Excitation) | ✓ | |
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| Observation Method > Fluorescence (Ultraviolet Excitation) | ✓ | |
| Observation Method > Differential Interference Contrast (DIC) | ✓ | |
| Observation Method > Phase Contrast | ✓ | |
| Observation Method > Brightfield | ✓ | |
| Focus > Motorized |
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| Focus > Z Drift Compensator | ✓ | |
| Observation Tubes > Widefield (FN 22) > Tilting Binocular | ✓ | |
| Observation Tubes > Widefield (FN 22) > Trinocular | ✓ | |
| Stage > Motorized | Contact your local sales representative to hear about motorized stage options | |
| Stage > Manual > Plain Stage | ✓ | |
| Stage > Mechanical > IX3-SVR Mechanical Stage with Right Handle |
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| Condenser > Motorized > Universal Condenser | NA 0.55/ W.D. 26.2mm | |
| Condenser > Manual > Universal Condenser | Dry: NA 0.9/ W.D. 1.5 mm, Oil: NA 1.4/ W.D. 0.63 mm (1.25 X - 100 X) | |
| Condenser > Manual > Ultra-Long Working Distance Condenser | NA 0.3/ W.D. 73.3 mm | |
| Dimensions (W × D × H) | 323 (W) x 475 (D) x 686 (H) mm (1 Deck Standard Configuration) | |
| Weight | 47 kg (1 Deck Standard Configuration) |
Applications
Light-induced Oscillatory/Sustained Expression of Ascl1 in Neural Progenitor Cells.
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For generating oscillatory expression of transgene, blue-light was illuminated for 1 min with a 3-hr interval, which maintaining proliferating NPCs.For achieving sustained expression of transgene, blue-light was illuminated for 2 min with a 30-min interval which promoting neuronal fate determination.
Image data courtesy of: Itaru Imayoshi and Ryoichiro Kageyama Institute for Virus Research, Kyoto University |
Reference material:
Itaru Imayoshi, Akihiro Isomura, Yukiko Harima, Kyogo Kawaguchi, Hiroshi Kori, Hitoshi Miyachi, Takahiro Fujiwara, Fumiyoshi Ishidate, and Ryoichiro Kageyama "Oscillatory Control of Factors Determining Multipotency and Fate in Mouse Neural Progenitors" Science 1242366 Published online 31 October 2013.
Synthetic ERK Activity Propagation by A Light-Switchable System
Reference:
Kazuhiro Aoki, Yuka Kumagai,Atsuro Sakurai,Naoki Komatsu,Yoshihisa Fujita,Clara Shionyu,Michiyuki Matsuda. "Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation" Molecular Cell,17 October 2013.
Real-time Imaging for Ring1BYFP/YFP ME
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Constitutive exchange of Ring1B-YFP at the PRC1 clusters. A PRC1 cluster in Ring1B YFP/YFP MEFs was subjected to photo-bleaching and subsequent sequential imaging.
Image data courtesy of: Kazuhiro Aoki and Michiyuki Matsuda Imaging Platform for Spatio-Temporal Information, Graduate School of Medicine, Kyoto University | |
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Constitutive exchange of Ring1B-YFP at the PRC1 clusters. A PRC1 cluster in Ring1B YFP/YFP MEFs was subjected to photo-bleaching and subsequent sequential imaging.
Image data courtesy of: Kyoichi Isono, and Haruhiko Koseki Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) |
Reference:
SAM domain polymerization links subnuclear clustering of PRC1 to gene silencing. Isono K, et al. Developmental Cell, 26, 565-577 (2013).
Brain Slice Culture Glia Cell
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Image data courtesy of:
Masafumi Minami Department of Pharmacology, Graduate School of Pharmaceutical Science, Hokkaido University |
Trans-mesenteric Migration of Enteric Neural Crest Derived Cells (ENCCs)
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Image data courtesy of:
Hideki Enomoto Neuronal Differentiation and Regeneration, RIKEN CENTER FOR DEVELOPMENT BIOLOGY |
Resources
Related Components
Components
- Spatial and Temporal Precision
- Perfect Reproducibility
- Flexibility
- Cell surface imaging with high Z-resolution
- Minimal background noise
- Wide choice of excitation wavelength
- True multi-color TIRFM
- Software adjustment of penetration depths
- Ease of use
- Perfect control of all environmental conditions
- First complete thought-out workflow-oriented incubation solution
- Open access
- Always in Focus
- Designed for Ease-of-use
- Dedicated to Live Cell Imaging
- High-Precision, Multi-Area Imaging with cellSens Software
Color Cameras
Monochrome Cameras
Electron multiplying CCD cameras amplify optical signals without increasing noise. They can capture high-speed images of dim specimens and perform well for fluorescence live-cell imaging with reduced intensity excitation light. They are used to observe protein interaction within a cell and for real-time imaging.
- CCD technology for detecting ultra-low light
- Dedicated, ultrasensitive scientific camera platform
- Quantifiable and reproducible imaging
- 1.4-megapixel monochrome cooled CCD
- Low noise and high sensitivity
- Fast Live image speed
Software
Providing intuitive operations and a seamless workflow, cellSens software’s user interface is customizable so you control the layout. Offered in a range of packages, cellSens software provides a variety of features optimized for your specific imaging needs. Its Graphic Experiment Manager and Well Navigator features facilitate 5D image acquisition. Achieve improved resolution through TruSight™ deconvolution and share your images using Conference Mode.
- Improve experiment efficiency with TruAI™ deep-learning segmentation analysis, providing label-free nuclei detection and cell counting
- Modular imaging software platform
- Intuitive application-driven user interface
- Broad feature set, ranging from simple snapshot to advanced multidimensional real-time experiments
Advanced Imaging Solutions (systems)
Light Sources
- Six independent solid state light sources
- Manual change of filters for different bandpasses
Facilitating fluorescence applications, this light-guide-coupled illumination system reduces thermal effects and vibration. It is easy to install and delivers excellent long- and short-term stability. A six-step iris effectively controls intensity and enables simple, staged intensity adjustment, and its pre-centered burner reduces running costs.
- Powerful 130 W burner with long lifetime
- Stable and uniform light output
- Even alignment-free illumination
- Incorporates up to four high power solid state LEDs
- 365, 385, 405, 447, 460, 525, 635, 660 and 735 nm available
- Ultra-fast switching times in microsecond range