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FV4000

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FV4000

Transforming Precision Imaging

Empower Your Confocal Microscopy Imaging Experiments

Transform your images with the new FLUOVIEW™ FV4000 confocal laser scanning microscope. Advanced imaging technology enables higher precision images to empower researchers with more reliable data from their samples. With our breakthrough SilVIR™ detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. With the FV4000 confocal microscope, researchers can acquire higher-quality, quantitative image data in less time and with less effort.

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Experience the systems innovations, including:

  • Game-changing dynamic range for imaging from the macro scale to subcellular structures
  • Multiplex up to six channels simultaneously with TruSpectral™ technology
  • Redesigned high-speed, high-resolution scanners for fixed and live cell imaging
  • Improved depth and photosensitivity with pioneering near-infrared (NIR) capabilities and renowned optics
  • Peace of mind with the reliable, repeatable SilVIR detector
  • Industry-leading* ten laser lines with a broader spectral range from 405 nm to 785 nm
  • Modular design to meet researchers’ ever-changing needs, including the ability to upgrade to multiphoton imaging on a single system

*As of October 2023.

Easy-to-Acquire, Quantitative Confocal Data

The FV4000 confocal microscope uses our advanced, silicon-based SilVIR™ detector that makes it easier than ever to acquire precise, reproducible data.

SilVIR Next-Generation Detector Technology

The SilVIR detector combines two advanced technologies—a silicon photomultiplier (SiPM) and our patented* fast signal processing design.

  • High dynamic range
  • Low noise
  • No degradation of sensitivity
  • Less sensitivity variation among other detectors

*Patent number US11237047

Learn more about the SilVIR detector
 

40x Brain Stitch

Neurofilament-heavy chain (NFH) in green, myelin basic protein (MBP) in red, glutathione S-transferase pi 1 (GSTpi) in blue. Mouse cerebellum captured with a UPLXAPO40X objective.
Sample courtesy of Katherine Given, Ph.D. Principal Investigator, Neurobiology University of Colorado Anschutz Medical Campus, Aurora, Colorado

Conventional detector

SiLVIR detector

The histogram on the image captured using the SilVIR detector shows a discrete pattern where the intensity can be converted to the photon number. The detector’s fluorescence intensity can be quantified as the photon number, and the background level is extremely low.

More Information from Your Confocal Images

The system’s updated TruSpectral technology combined with high sensitivity SilVIR detectors enable you to see more by making it possible to multiplex up to six channels simultaneously.

Stictch A01 GOO1 Projection Z

The neurovascular unit of a mouse hippocampus. Blue; DAPI cell nuclei, Green; GFAP AF488. Astrocytes, yellow; DsRed pericytes, magenta; collagen IV AF647 basement membrane of blood vessels, gray; AQ-4. Astrocytes water channel.
Sample courtesy of: Hiroshi Hama and Atsushi Miyawaki, Cell Function Dynamics, RIKEN CBS.

CytoSkelton 4 color cell

Cytoskeleton sample: HeLa cells stained with DAPI (blue), Pericentrin (Centrosome, green), a-Tubulin (Microtubules, Alexa-568; red) and phalloidin (Actin, Alexa-647; magenta).
Sample Courtesy of: Sample preparation Alexia Ferrand; sample acquisition Sara R. Roig and Alexia Ferrand. Imaging Core Facility, Biozentrum, University of Basel.

Flexible Macro to Micro Imaging

The macro-to-micro workflow enables you to easily observe the target sample from the macro level—whole body or tissue—down to the cell or subcellular level.

Overview image of a Drosophila wing

Edge image of a Drosophila wing

Overview and edge images of a Drosophila wing (42-hour pupation). Stained with phalloidin (AlexaFluor 405, F-actin, Cyan), anti-phosphotyrosine antibody (AlexaFluor 555, cell surface, red), and anti-HRP antibody (AlexaFluor 647, axon, blue). Sample courtesy of: Sun Zhengkuan, Shigeo Hayashi, Laboratory for Morphogenetic Signaling, RIKEN Center for Biosystems Dynamics Research, Japan.

Gentler High-Speed Time-Lapse Confocal Imaging

HeLa cells labeled by MitoView 720. XYZT imaging by 1K resonant scanner for 30 min.

Time-lapse imaging is easier with smart features:

  • Capture every moment of live cell dynamics: resonant scanner can acquire high-resolution images over a wide area
  • Minimal phototoxicity: the scanner’s short pixel dwell time reduces the time the focused laser beam rests on a single spot
  • Better signal-to-noise ratio: the SilVIR detector’s high sensitivity produces higher quality images at higher speeds
  • Greater precision: rolling average processing maintains qualifications and time resolution

Reproducible Image Data Between Users and Systems

The SilVIR detector has less sensitivity loss over time compared to previous-generation detector technologies. With our laser power monitor (LPM) and TruFocus™ Z-drift compensator, achieve reproducible images under consistent conditions for better reproducibility. Different users on different days can acquire the same precise images using the same settings. Even the images acquired by different FV4000 microscopes can be compared and discussed using the same photon number intensity scale.

Microscope Support and Service You Can Count On

We designed the FV4000 system to be easy to maintain:

  • Semiconductor-based SilVIR detector is stable and durable
  • Laser power monitor continuously checks the illumination condition and makes adjustments to maintain consistent laser power
  • System admin can view log files to keep track of the service maintenance schedule

We stand behind our products with a commitment to fast service and technical support. We offer various support plans to keep your microscope running at peak performance at a predictable cost as well as remote support options, so you don’t need to wait for an engineer or specialist to visit if you’re having an issue.

Microscope Support and Service You Can Count On

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