Multichannel Fluorescence

• Observe samples with multiple stains and in combination with other imaging modes like phase contrast or gradient contrast.
• Capacity of up to eight mirror cubes to accommodate many experimental conditions.
• Capture images under optimal conditions quickly with automated exposure time and Z offset calibrations for each channel.
• Neatly displays merged images to ensure each acquisition matches your standards.

_{BPAE cells. Stain: Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, DAPI.}

Rat cerebellum labeled using 5 fluorochromes

Easily acquire multichannel fluorescence images with wavelengths ranging from UV to NIR.

_{Stains used: Hoechst (blue), GFAP (green), MAP2 (orange), Calbindin (red), MBP (magenta).}

_{Objective: UPLXAPO4X}

Stitching

• Capture entire tissue samples or assess the condition of cell culture flasks over wide areas rapidly at high resolution.
• High-accuracy stitching makes the joints between images nearly invisible in both brightfield and fluorescence acquisition modes.
• Even tilted or uneven samples can be cleanly stitched together.

_{Mouse lung captured with UPLXAPO4X objective. Stain: HE.}

Z-Stack

• Acquire multiple images in the Z direction to accommodate thick samples.
• Create all-in-focus images with just a few clicks.
• Achieve sharp, blur-free images using TruSight™ 3D deconvolution.

_{Subcellular localization of kendrin/pericentrin, centrosome protein. Image data courtesy of Kazuhiko Matsuo, Ph.D., Division of Developmental Biology and Anatomy, Department of Anatomy, Kyoto Prefectural University of Medicine.}