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The proximity between the mitochondrial and F-actin networks was visualized for this image by treating a culture of bovine pulmonary artery endothelial cells with the mitochondrion-selective stain MitoTracker Red CMXRos and BODIPY FL conjugated to a phallotoxin (phalloidin). The far-red fluorescent DNA probe DRAQ5 was utilized as a nuclear counterstain.
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