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The heart of the FLUOVIEW FV1200 laser scanning microscope is the scanning unit itself. Our new galvanometer mirrors feature an innovative silver coating that deliver outstanding reflectance across a broad range of wavelengths. The integrated spectral-type detector unit includes two independent spectral detection channels, each configured with a diffraction grating and variable slit for high resolution wavelength separation and high speed bandwidth selection. This enables unmatched 2 nm wavelength resolution at high precision and high speed.
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The design of this detector unit is unique as it integrates the scanning unit, tube lens and pupil projection lens into a single compact unit. Use of the microscope fluorescence illuminator light path ensures the highest coupling efficiency without limiting the expandability of the microscope itself. Visible, UV and IR laser introduction ports are featured.
Anti-oxidization silver coating ensures durability and improves reflection efficiency in both excitation and emission with the set of two galvanometer scanning mirrors, increasing the reflectance in the visible range by 5 - 15%, and IR reflectance up to 22%.
The original spectral detection system features two independent spectral detection channels, each configured with a diffraction grating and variable slit for high resolution wavelength separation and high speed bandwidth selection. Accurate spectral unmixing of overlapping fluorescence emissions becomes possible with a linear resolution of 2 nm throughout the visible spectrum (400 to 800 nm). Two different spectral deconvolution algorithms – normal and blind deconvolution – allow an easy and fast separation of different but overlapping fluorochromes within the specimen. High speed spectroscopy can be performed at a maximum speed of 100 nm/msec.
With the spectral detection system, the ideal wavelength spectrum is automatically selected once a fluorochrome combination has been selected using the software. Manual adjustment of the wavelength range is also possible according to the fluorescence emission wavelength peaks.
The incorporation of highly stable galvanometer scanner mirrors and an Olympus original acceleration circuit enables high-speed imaging of 16 fps for a 256x256 image format or 38 fps for smaller image formats.
Conventional raster scanning cannot always complete the photobleaching process quickly. The unique Tornado scanning ability of our SIM unit makes the procedure much more efficient and significantly reduces the necessary bleaching or activation time.
Laser light intensity is monitored and fed back to the acousto-optic tunable filters (AOTFs), helping to stabilize the excitation light during long-term (overnight) time lapse imaging.
Special high performance multi-alkali PMT selection in this scanning unit ensures high detection efficiency using reliable and well-proven PMT technology.
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