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Anwendungsbeispiele

Support Biopsy Efficiency with the Simplified Operation of the IX73 Inverted Microscope


The Challenge

With objectives of lowering the miscarriage rate and improving the implantation rate, preimplantation genetic testing (PGT) is rising in popularity worldwide; therefore, the number of embryo biopsies performed is increasing accordingly.

In an embryo biopsy, some cells are removed from the embryo under an inverted microscope using relief contrast observation. The appropriate contrast adjustment is required to determine the location to irradiate the laser and the suction the cells, but complex microscope operations can cause user fatigue.

Olympus’ Solution to Improve the Efficiency of the Biopsy Workflow

Olympus’ relief contrast enables observation of 3-dimensional oocytes in plastic dishes. Since the relief contrast imaging is unaffected by the polarizing effect of the plastic dish, you can clearly view the oocyte zona pellucida to check its condition.

In addition, the dedicated remote hand switch of the IX73 semi-motorized system makes it easy to achieve suitable contrast and magnification/condenser settings with the push of a button. Streamlining the microscope operation to a minimum enables users to concentrate on the delicate biopsy manipulations, which require careful attention. Since the motorized operation using the hand switch reduces user intervention above the stage, it also decreases the risk of accidentally touching the sample or needle of the injector.

Remote hand switch

Remote hand switch

Samples and images data are courtesy of Cooper Surgical Inc.

Samples and images data are courtesy of Cooper Surgical Inc.

Summary of the IX73 Semi-Motorized System’s Advantages for Embryo Biopsies

The IX73 system’s remote hand switch simplifies and streamlines microscope operations, supporting reliable and efficient embryo biopsies. The improved microscope operation workflow helps you manage the increased embryo biopsy workload.
 

Comment by Dr. Watanabe

When performing an embryo biopsy using the IX73 semi-motorized system, I am impressed by the smooth control of the magnification and laser system with the dedicated hand switch. In the past, when changing the magnification, we had to touch the condenser and objective lens, which required careful operation. I, like many users, have experienced shaking the microscope by accidentally touching it too forcefully. The IX73 semi-motorized system enables you to perform these operations by lightly pressing a switch without touching the microscope, so you can focus more on the embryo biopsy itself.

This application note was prepared with the help of the following researcher:
Dr. Hideaki Watanabe
Sugiyama Clinic Shinjuku
Center for Reproductive Medicine and Implantation Research

IX73 SL-ICSI

IIX3-ICSI/IMSI inverted microscope

Verwendete Produkte

ICSI-/IMSI-Plattform

IX3-ICSI/IMSI

Das IX3-ICSI System wurde entwickelt, um die Anwendung der intrazytoplasmatischen Spermieninjektion (ICSI) als Teil des In-vitro-Fertilisationsverfahrens (IFV) zu erleichtern. Das Relief-Kontrastverfahren von Olympus ermöglicht die 3D-Oozytenbetrachtung in Kunststoffschalen zur Überprüfung des Zustands der Zona pellucida. Das teilmotorgesteuerte IX3-System verfügt über einen speziellen Handschalter für ICSI, mit dem die Betrachtungsmethode und die Vergrößerung auf Tastendruck geändert werden können. Die Polarisationsmethode dient der Betrachtung der meiotischen Spindel und ihrer Position, um so die Reife der Eizelle zu bestätigen und eine Beschädigung während der Injektion zu vermeiden, während die intrazytoplasmatische morphologisch selektierte Spermieninjektion mit der DIC-Methode (differenzieller Interferenzkontrast) durchgeführt wird.

  • Optimiert für einen effektiveren Ablauf der ICSI
  • Einfache Überprüfung des Zustands der Eizelle durch Spindelvisualisierung
  • Optimierte ICSI-Schritte durch motorgesteuerte Bedienung

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