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Overview
 | Olympus UIS2 fluorescence mirror units are optimized to maximize fluorophore performance, producing uniform and highly efficient light transmittance. The filter efficiency enables the use of weaker excitation light to minimize photobleaching and sample damage. |
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Filter Cubes for BX3, IX3 Microscopes, and APX100 system |
| Unit Name | Excitation Filter | Emission Filter | Dichromatic Mirror | |
|---|---|---|---|---|
| Ultraviolet Excitations | Loading gallery... Loading... | 340-390 | 420IF | 410 |
Loading gallery... Loading... | 360-370 | 420IF | 410 | |
Loading gallery... Loading... | 360-370 | 420-460 | 410 | |
| Blue Violet Excitations | Loading gallery... Loading... | 400-440 | 460IF | 455 |
| Blue Excitations | Loading gallery... Loading... | 460-495 | 510IF | 505 |
Loading gallery... Loading... | 460-495 | 510-550 | 505 | |
Loading gallery... Loading... | 470-495 | 510IF | 505 | |
Loading gallery... Loading... | 470-495 | 510-550 | 505 | |
| Green Excitations | Loading gallery... Loading... | 530-550 | 575IF | 570 |
Loading gallery... Loading... | 530-550 | 575-625 | 570 | |
Loading gallery... Loading... | 540-550 | 575-625 | 570 | |
| Yellow Excitations | Loading gallery... Loading... | 540-585 | 600IF | 595 |
| Fluorescent Protein Variants | Loading gallery... Loading... | 425-445CFP | 460-510CFP | 455CFP |
Loading gallery... Loading... | 460-480GFP | 495-540GFP | 490GFP | |
Loading gallery... Loading... | 490-500YFP | 515-560YFP | 515YFP | |
Loading gallery... Loading... | 535-555HQ | 570-625HQ | 565HQ | |
Loading gallery... Loading... | 565-585 | 600-690 | 595 |
Filter Cubes for IX3 Microscopes |
| Unit Name | Excitation Filter | Emission Filter | Dichromatic Mirror | |
|---|---|---|---|---|
| Ultraviolet Excitations | Loading gallery... Loading... | 340-390 | 420IF | 410 |
| Blue Violet Excitations | Loading gallery... Loading... | 400-440 | 460IF | 455 |
| Green Excitations | Loading gallery... Loading... | 530-550 | 575IF | 570 |
| Fluorescent Protein Variants | Loading gallery... Loading... | 425-445CFP | 460-510CFP | 455CFP |
Loading gallery... Loading... | 460-480GFP | 495-540GFP | 490GFP | |
Loading gallery... Loading... | 490-500YFP | 515-560YFP | 515YFP | |
Loading gallery... Loading... | 565-585 | 600-690 | 595 |
Filter Cubes for IX2, BX2, and MVX10 Microscopes |
| Unit Name | Excitation Filter | Emission Filter | Dichromatic Mirror | |
|---|---|---|---|---|
| Ultraviolet Excitations | Loading gallery... Loading... | 330-385 | 420 | 400 |
Loading gallery... Loading... | 360-370 | 420 | 400 | |
Loading gallery... Loading... | 360-370 | 420-460 | 400 | |
| Violet Excitations | Loading gallery... Loading... | 400-410 | 455 | 455 |
| Blue Violet Excitations | Loading gallery... Loading... | 400-440 | 475 | 455 |
Loading gallery... Loading... | 420-440 | 475 | 455 | |
| Blue Excitations | Loading gallery... Loading... | 420-480 | 520IF | 500 |
Loading gallery... Loading... | 460-490 | 520IF | 500 | |
Loading gallery... Loading... | 470-490 | 520IF | 500 | |
Loading gallery... Loading... | 460-495 | 510IF | 505 | |
Loading gallery... Loading... | 460-495 | 510-550 | 505 | |
Loading gallery... Loading... | 470-495 | 510IF | 505 | |
Loading gallery... Loading... | 470-495 | 510-550 | 505 | |
| Green Excitations | Loading gallery... Loading... | 510-550 | 590 | 570 |
Loading gallery... Loading... | 530-550 | 590 | 570 | |
Loading gallery... Loading... | 530-550 | 575IF | 570 | |
Loading gallery... Loading... | 530-550 | 575-625 | 570 | |
Loading gallery... Loading... | 540-550 | 575-625 | 570 | |
| Yellow Excitations | Loading gallery... Loading... | 545-580 | 610IF | 600 |
| Fluorescent Protein Variants | Loading gallery... Loading... | 425-445HQ | 460-510HQ | 450 |
Loading gallery... Loading... | 460-480HQ | 495-540HQ | 485 | |
Loading gallery... Loading... | 490-500HQ | 515-560HQ | 505 | |
Loading gallery... Loading... | 535-555HQ | 570-625HQ | 565 |
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Selection Chart
Fluorescent Protein Variants |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| AmCyan | 458 | 489 | U-FCFP, U-FBVW | U-MWBV2, U-MNBV2, U-MCFPHQ |
| Azami-Green | 492 | 505 | U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN | U-MWIB3, U-MWIBA3, U-MNIB3, U-MNIBA3, U-MGFPHQ |
| DsRed2 | 563 | 582 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| EBFP | 380 | 440 | U-FUNA, U-FUW, U-FUN | U-MWU2, U-MNU2, U-MNUA2, U-MNV2 |
| ECFP | 433 | 475 | U-FCFP, U-FBVW | U-MCFPHQ, U-MWBV2, U-MNBV2, U-MNV2 |
| EGFP | 488 | 507 | U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN | U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3 |
| EYFP | 513 | 527 | U-FBWA, U-FBNA, U-FYFP, U-FBW, U-FBN | U-MYFPHQ, U-MWIBA3, U-MNIBA3, U-MWIBA3, U-MNIBA3, U-MNIB3, U-MNB2, U-MWB2, U-MWIB3 |
| Fucci (mAG1) | 492/551 | 505/565 | U-FBNA, U-FGFP, U-FBWA, U-FBW, U-FBN | U-MNIBA3, U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3 |
| GFP (S65T) | 488 | 510 | U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN | U-MGFPHQ, U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3 |
| GFP (WT) | 395, 475 | 510 | U-FBWA, U-FBNA, U-FGFP, U-FBW, U-FBN | U-MWIBA3, U-MNIBA3, U-MGFPHQ, U-MWIB3, U-MNIB |
| HcRed | 588 | 618 | U-FYW | U-MWIY2 |
| Midoriishi-Cyan | 472 | 495 | U-FBWA, U-FBW, U-FBNA, U-FBN, U-FCFP, U-FGFP | U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3, U-MCFPHQ, U-MGFPHQ |
| mCherry | 580 | 610 | U-FMCHE, U-FYW | U-MWIY2 |
| mRFP | 584 | 607 | U-FMCHE, U-FYW | U-MWIY2 |
| mStrawberry | 574 | 596 | U-FMCHE, U-FYW | U-MWIY2 |
| RFP (DsRed) | 558 | 583 | U-FRFP, U-FGW, U-FGWA, U-FGNA | U-MRFPHQ, U-MWIG3, U-MWIGA3, U-MNIGA3 |
| Venus | 515 | 528 | U-FYFP | U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MSWB2, U-MWB2, U-MWIB3, U-MWIBA3 |
Photoactivation |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Dronpa-Green | 503 | 518 | U-FBN, U-FBNA, U-FBW, U-FBWA, U-FYFP | U-MGFPHQ, U-MNB2, U-MNIB3, U-MNIBA3, U-MWB2, U-MWIB3, U-MWIBA3, U-MYFPHQ |
| PA-GFP | 504 | 517 | U-FGFP, U-FBWA, U-FBNA, U-FBW, U-FBN | U-MGFPHQ, U-MNIB3, U-MWIBA3, U-MWIB3, U-MNIBA3 |
Photoconversion |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Kaede (Green/Red) | 509/573 | 518/582 |
U-FBN, U-FBNA, U-FBW, U-FBWA, U-FGFP,
U-FYFP, U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIB3, U-MWIBA3, U-MGFPHQ, U-MYFPHQ, U-MNB2, U-MNIB3, U-MNIBA3,U-MWB2, U-MNIGA3, U-MRFPHQ, U-MWIG3, U-MWIGA3 |
Carbohydrate Modification, Protein Modification (Labeling) |
Protein modification |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Alexa Fluor 350 | 346 | 442 | U-FUNA, U-FUW, U-FUN | U-MNUA2, U-MWU2, U-MNU2 |
| Alexa Fluor 405 | 402 | 421 | U-FUNA, U-FUW, U-FUN | U-MNUA2, U-MWU2, U-MNU2 |
| Alexa Fluor 430 | 434 | 539 | U-FBVW | U-MWBV2, U-MNBV2, U-MNV2 |
| Alexa Fluor 488 | 495 | 519 | U-FBWA, U-FBNA, U-FBW, U-FBN | U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3,UMNB2, U-MWB2 |
| Alexa Fluor 532 | 531 | 554 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| Alexa Fluor 546 | 556 | 573 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| Alexa Fluor 555 | 555 | 567 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| Alexa Fluor 568 | 578 | 603 | U-FGW, U-FGWA, U-FGNA, U-FRFP, U-FYW, U-MCHE | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ, U-MWIY2 |
| Alexa Fluor 594 | 590 | 617 | U-FYW, U-FMCHE | U-MWIY2 |
| Allophycocyanin (APC) | 650 | 660 | U-FYW | U-MWIY2 |
| 7-amino-4-methylcoumarin-3-acetic acid (AMCA) | 349 | 448 | U-FUNA, U-FUW, U-FUN | U-MNUA2, U-MWU2, U-MNU2 |
| BODIPY FL | 505 | 513 | U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP | U-MWIBA3, U-MWIB3, U-MNIBA3, U-MNIB3 |
| Cascade Blue | 376 | 425 | U-FUNA, U-FUW, U-FUN | U-MNUA2, U-MWU2, U-MNU2 |
| Fluorescein-isothiocyanate (FITC) | 494 | 518 | U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP | U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ |
| Oregon Green 488 | 496 | 524 | U-FBWA, U-FBW, U-FBNA, U-FBN, U-FGFP, U-FYFP | U-MWIBA3, U-MNIBA3, U-MWB2, U-MNB2, U-MWIB3, U-MNIB3, U-MGFPHQ, U-MYFPHQ |
| Phycoerythrin B (PE-B) | 545 | 576 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| Phycoerythrin R (PE-R) | 490, 565 | 578 | U-FGNA, U-FGW, U-FGWA, U-FRFP | U-MNIGA3, U-MWIG3, U-MWIGA3, U-MRFPHQ |
| Rhodamine B-isothiocyanate (RITC) | 543 | 580 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| Texas Red | 595 | 615 | U-FYW, U-MCHE | U-MWIY2 |
| Tetramethylrhodamine-isothyocyanate (TRITC) | 541 | 572 | U-FGW, U-FGWA, U-FGNA, U-FRFP | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MRFPHQ |
| 1-Anilinonaphthalene-8-sulfonic acid (ANS) | 365 | 460 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| CPM (7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin) | ~384 | 470 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| Cy2 | 489 | 506 | U-FBWA, U-FBNA, U-FBW, U-FBN | U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3 |
| Cy3 | (512); 550 | 570; (615) | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3 |
| Cy3.5 | 581 | 594; (640) | U-FYW, U-FMCHE | U-MWIY2 |
| Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) | 340 | 525 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| Dansyl chloraide | 360 | 520 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| Dansyl hydrazine | 340 | 525 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| Eosin | 525 | 545 | U-FBWA, U-FBNA, U-FBW, U-FBN | U-MWIBA3, U-MNIBA3, U-MWIB3, U-MNIB3 |
| Fluorescamine | 390 | 465/475 | U-FUW, U-FUN, U-FNUA | U-MWU2, U-MNU2, U-MNUA2, U-MNV2 |
| FluorX | 494 | 520 | U-FBWA, U-FBNA, U-FBW, U-FBN | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB2, U-MWB2, U-MNB2 |
| Lucifer Yellow CH | 425 | 528 | U-FBVW, U-FBW, U-FBW, U-FBWA | U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2 |
| Monochlorobimane (mBCI) | 394 | 490 | U-FUW, U-FUN, U-FUNA | U-MNUA2, U-MWU2, U-MNU2, U-MNV2 |
| o-phtalaldehyde (OPT) | 340 | 455 | U-FUNA, U-FUW, U-FUN | U-MNUA2, U-MWU2, U-MNU2 |
| Resorufin | 572 | 583 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2 |
| SITS (Primuline) | 395-425 | 450 | U-FUW, U-FUN, U-FUNA | U-MNUA2, U-MWU2, U-MNU2, U-MNV2 |
| SITS (Stilbene Isothiosulphonic acid) | 365 | 460 | U-FUW, U-FUN, U-FUNA | U-MNUA2, U-MWU2, U-MNU2 |
Carbohydrate modification |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acrifl avine | 436 | 520 | U-FBVW, U-FBW | U-MWBV2, U-MNBV2, U-MWB2, U-MSWB2 |
Nuclei, Nucleic Acid Staining |
Nuclei, nucleic acid |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acridine Orange | 500 | 530/630-650 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Acridine Yellow | 470 | 550 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2 |
| Acrifl avine-Feulgen | 455 | 515 | U-FBVW, U-FBW, U-FBWA | U-MWBV2, U-MNBV2, U-MWIB3, U-MWIBA3, U-MWB2, U-MSWB2 |
| Auramine O-Feulgen | 460 | 550 | U-FBW, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2 |
| 7-Amino Actinomycin D | 546 | 647 | U-FYW, U-FGW, U-FMCHE | U-MWIG3, U-MWG2, U-MNG2, U-MWIY2 |
| bis-Aminophenyl-oxadizole (BAO) -Feulgen | 380 | 470 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| BOBO 1 | 462 | 481 | U-FBVW | U-MWBV2, U-MNBV2, U-MCFPHQ |
| BO PRO 1 | 462 | 481 | U-FBVW | U-MWBV2, U-MNBV2 |
| Chromomycin A3 | 445 | 575 | U-FBVW | U-MWBV2, U-MNBV2, U-MSWB2 |
| 4, 6-diamidino-2-phenyl-indole HCI (DAPI) | 345 | 455 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Ethidium Bromide | 493 | 620 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2 |
| Hoechst 33258 | 345 | 478 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Hoechst 33342 | 343 | 483 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Mithramycin | 445 | 575 | U-FBVW | U-MWBV2, U-MNBV2, U-MSWB2 |
| Olivomycin | 430 | 545 | U-FBVW | U-MWBV2, U-MNBV2 |
| Pararosaniline-Feulgen | 570 | 625 | U-FYW, U-FMCHE, U-FGW, U-FGWA, U-FGNA | U-MWIY2, U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3 |
| POPO 1 | 434 | 456 | U-FBVW, U-FCFP | U-MNV2, U-MWBV2, U-MNBV2, U-MCFPHQ |
| Propidium Iodide (PI) | 536 | 617 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
| Pyronin Y | 540 | 570 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3 |
| Quinacrine Mustard | 423 | 503 | U-FBVW | U-MWBV2, U-MNBV2 |
| SYTOX Green nucleic acid stain | 504 | 523 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Thiazole Orange | 510 | 530 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| TOTO 1 | 509 | 533 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| TOTO 3 | 642 | 661 | U-FYW, U-FMCHE | U-MWIY2 |
| TO PRO 3 | 642 | 661 | U-FYW, U-FMCHE | U-MWIY2 |
| YO PRO 1 | 491 | 509 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| YOYO 1 | 491 | 509 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FGFP, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
Cell Viability Assessment |
Living cell staining |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acridine Orange | 500 | 530/630-650 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| BCECF indicator |
482(low pH)
503(high pH) |
520(low pH)
528 (high pH) | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Calcein | 494 | 517 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Carboxyfl uorescein diacetate (CFDA) | 496 | 515 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Fluorescein diacetate (FDA) | 496 | 515 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
Dead cell staining |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Ethidium Bromide | 493 | 620 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2, U-SWG2 |
| Ethidium homodimer-1 | 528 | 617 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3 |
| Propidium Iodide (PI) | 536 | 617 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
Membranes, Organelle, Membrane Potential |
Membranes |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| 8-Anilino-1-naphthalene sulfonic acid (1,8-ANS) | 385 | 485 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Dil | 550 | 565 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3 |
| DiO | 484 | 501 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2 |
| Diphenylhexatriene (DPH) | 351 | 430 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Filipin | 350 | 480 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Fluorescamine | 390 | 465 | U-FUW, U-FUN | U-MWU2, U-MNU2, U-MNV2 |
| Merocyanine 540 | 500 | 580 | U-FGW | U-MWG2, U-MNG2, U-MWIG3 |
Organelle |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acridine Orange | 500 | 530/630-650 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| BODIPY FL Ceramide | 503 | 512 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3,U-MNIB3, U-MWIBA3, U-MNIBA3 |
| DiO C6(3) | 484 | 500 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2 |
| ER Tracker Blue-White DPX | 374 | 575 | U-FUW, U-FUN | U-MWU2, U-MNU2, U-MNV2 |
| JC-1 | 485 | 530 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MSWB, U-MWB2, U-MNB2 |
| Lyso Tracker Red DND-99 | 577 | 590 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
| Mito Tracker Green | 490 | 516 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Mito Tracker Red CMXRos | 579 | 599 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
| Nile Red (phospholipid) | 552 | 636 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWG2, U-MNG2, U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
| Rhodamine 123 | 507 | 530 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Pyronin Y | 540 | 570 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2 |
Membrane potential |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Di-4-ANEPPS | 497 | 710 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| Di-8-ANEPPS | 465 | 635 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| FM 1-43 | 473 | 579 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| RH160 | 577 | >610 | U-FGW, U-FGWA, U-FGNA, U-FYW | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWIY2 |
| RH414 | 490 | >662 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| RH421 | 541 | >590 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3 |
| RH423 | 520-550 | >610 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3 |
| RH795 | 560 | 650 | U-FGW, U-FYW | U-MWIG3, U-MWIY2 |
Bacteria Staining, Bone Staining |
Bacteria |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acridine Orange | 500 | 530/630-650 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Acridine Yellow | 470 | 550 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3, U-MWB2, U-MNIB2, U-MSWB2 |
| Auramine O | 460 | 550 | U-FBVW, U-FBW | U-MWBV2, U-MNBV2, U-MSWB2, U-MWB2, U-MWIB, U-MWIB3 |
| Berberine sulfate | 430 | 550 | U-FBVW | U-MWBV2, U-MNBV2 |
| Coriphosphine O | 460 | 575 | U-FBW | U-MWB2, U-MWIB3 |
| CTC | 430, 480 | 630 | U-FBVW, U-FBW, U-FBN | U-MWBV2, U-MNBV2, U-MWIB3, U-MNIB3 |
| Hoechst 33258 | 365 | 465 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
Bone |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Acidic Fuchsin (Pararosaniline) | 540 | 630 | U-FGW, U-FGN | U-MWG2, U-MNG2, U-MWIG3 |
| Calcein | 495 | 520 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Calcein Blue | 375 | 435 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Tetracycline | 390 | 560 | U-FUW, U-FBVW | U-MWU2, U-MNV2, U-MWBV2, U-MNBV2 |
| Xylenol Orange | 440, 570 | 610 | U-FGW, U-FGN | U-MWG2, U-MNG2, U-MWIG3 |
Ion Concentrations |
Calcium |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| BTC | 400/480 | 540 | U-FBN, U-FBNA, U-FBW, U-FBWA | U-MNV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
| Calcium Green | 505 | 532 | U-FBN, U-FBNA, U-FBW, U-FBWA | U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
| Fluo 4 | 495 | 510 | U-FBN, U-FBNA, U-FBW, U-FBWA | U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
| Oregon Green 488 BAPTA-1 | 494 | 523 | U-FBN, U-FBNA, U-FBW, U-FBWA | U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
| Oregon Green 488 BAPTA-5N | 494 | 521 | U-FBN, U-FBNA, U-FBW, U-FBWA | U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
pH |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| BCECF | 440/490 | 530 | U-FBVW, U-FBN, U-FBNA, U-FBW, U-FBWA | U-MWBV, U-MNIB3, U-MNIBA3, U-MWIB3, U-MWIBA3 |
Nerve Cell Tracer, Others |
Nerve cell tracer |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Evans Blue | 470-540 | 680 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| Fast Blue | 360 | 410 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| Granular Blue | 375 | 410 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
| True Blue | 373 | 404 | U-FUW, U-FUN, U-FUNA | U-MWU2, U-MNU2, U-MNUA2 |
Others |
| Excitation Maximum (nm) | Emission Maximum (nm) | Applicable BX3/IX3/APX100 Mirror Unit | Applicable BX2WI/MVX10 Mirror Unit | |
|---|---|---|---|---|
| Aniline blue | 370 | 509 | U-FUW, U-FUN | U-MWU2, U-MNU2 |
| Alexa Fluor 488-Phalloidin | 495 | 518 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Calcofl uor white | 440 | 500-520 | U-FUW, U-FUN | U-MWU2, U-MNU2, U-MNV2 |
| Euchrysine (Acridine Orange) | 500 | 530/630-650 | U-FBW, U-FBN, U-FBWA, U-FBNA, U-FYFP | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| NanoOrange | 485 | 590 | U-FBW, U-FBN | U-MWIB3, U-MNIB3 |
| NBD-Phallacidin | 465 | 536 | U-FBW, U-FBN, U-FBWA, U-FBNA | U-MWIB3, U-MNIB3, U-MWIBA3, U-MNIBA3 |
| Rhodamine-Phalloidin | 540 | 565 | U-FGW, U-FGWA, U-FGNA | U-MWIG3, U-MWIGA3, U-MNIGA3, U-MWG2, U-MNG2 |
| sulforhodamine 101 | 586 | 605 | U-FYW, U-FMCHE | U-MWIY2 |
| Thiofl avin T | 430 | 485 | U-FBVW | U-MWBV2, U-MNBV2 |
